FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580272
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGCTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT38980.24666639667095283No Hit
GCATTATACTGTTGGCAGTAATAAATTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC30490.19294146830419065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28070.17762764891107355No Hit
AGTCAGGACATTGCCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGCAGGCCCCTAGGTCCCTGATCTATG27570.17446363663976833No Hit
GATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA23250.14712657061569145No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17380.10998106655056852No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC16870.1067537740338372No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16500.10441240495307137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16400.10377960249881032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16240.10276711857199267No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16160.10226087660858384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.041.4244881
GTATCAA59900.039.202971
GTGGTAT8950.037.5917661
TATCAAC68500.034.1657522
ATCAACG71900.032.6843
TCAACGC72250.032.4781844
CAACGCA73600.032.0263445
AACGCAG74050.031.9727486
TGGTATC10350.030.171592
ACGCAGA80500.029.2006077
CGCAGAG82150.028.7394248
AGAGTAC82750.028.44810111
CAGAGTA83750.028.02647610
GCAGAGT84850.027.7440249
AGTACGG61800.025.31897213
GAGTACG63100.025.07083312
CGGTCGA750.002044205322.90055520
AACGAAC18200.022.61244851
CAAACGA18200.022.57323549
ACGAACT16250.022.55103152
Produced by FastQC (version 0.11.5)