FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767515
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90211.175351621792408No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC10930.14240764024155877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA200.006168386454.10968858
GTGGTAT8800.045.08211
ATAGGCT307.152387E-444.8449519
GTATCAA60900.040.1900831
TATCAAC69700.035.141172
GGTATCA26700.034.2501451
TGAGTAC701.2041528E-633.5723534
TGGTATC11850.032.606262
TCAACGC77350.031.5107024
ATCAACG78000.031.338283
AACGCAG80450.030.5131026
CAACGCA80250.030.4636065
CGCAGAG85750.028.7465178
ACGCAGA85650.028.6624537
AGAGTAC88250.027.9303511
GCAGAGT88300.027.8783199
CGAACTA500.007709693627.61723139
CAGAGTA90100.027.35864410
CGGGCTA6650.026.29146817
AGTACGG60250.025.58128413
Produced by FastQC (version 0.11.5)