FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836515
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTT51400.6144540145723627No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50320.601543307651387No Hit
GTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA41480.49586678063154876No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32290.38600622822065356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31670.3785945260993527No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31390.3752473057865071No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30770.36783560366520623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29620.3540880916660191No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT28950.34607867163171013No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA28540.34117738474504344No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG27480.328505764989271No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT26110.3121282941728481No Hit
ATCCATAACTGTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC25550.30543385354715696No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25430.3039993305559374No Hit
CTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA25120.300293479495287No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA25100.30005439233008374No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24680.2950335618608154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24540.2933599517043926No Hit
GGTCTAGTCAGAGCCTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC24150.2886977519829292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22910.27387434774032743No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22660.27088575817528676No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21590.25809459483691266No Hit
CTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG21590.25809459483691266No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG21230.25379102586325414No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT20870.24948745688959553No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG20410.24398845208992068No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20290.24255392909870113No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20230.2418366676030914No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19820.23693538071642467No Hit
CTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC19730.23585948847301003No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCGTTCACTTTTGGCCAGGGGA19640.23478359622959544No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19460.23263181174276612No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19440.23239272457756285No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19410.232034093829758No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18890.2258178275344734No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18650.22294878155203432No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAAATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18600.22235106363902618No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18050.21577616659593674No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17880.21374392569170905No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACT17860.2135048385265058No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCC17650.21099442329187162No Hit
GTCTCCACTCTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA17430.20836446447463583No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA17370.20764720297902609No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17320.20704948506601795No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17260.2063322235704082No Hit
GGCCAAAAGTGAACGGAGTTTGTAGACCTTGCATGCAGTAATAAACCCCA17240.2060931364052049No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17210.20573450565740006No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16870.20167002384894472No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16590.19832280353609916No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16420.19629056263187153No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16100.19246516798861946No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG15320.18314076854569256No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14710.1758486100069933No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14540.17381636910276563No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14270.17058869237252172No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGACCGTCGCCC13920.16640466698146478No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13740.1642528824946355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13650.16317699025122084No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13490.1612642929295948No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC13340.1594711391905704No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13140.15708026753853785No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA13100.15660209320813137No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13060.15612391887772484No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT13030.15576528812991997No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12410.1483535860086191No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA12370.14787541167821258No Hit
GTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCC12370.14787541167821258No Hit
CTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCCCT12240.14632134510439143No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12080.1444086477827654No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12000.1434522991219524No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11890.1421373197133345No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATAACTGTGC11570.13831192507008241No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11470.13711648924406616No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11470.13711648924406616No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11420.13651877133105803No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11370.1359210534180499No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC11350.13568196625284662No Hit
GTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTA11310.13520379192244011No Hit
AGTCAGAGCCTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG11250.13448653042683037No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11250.13448653042683037No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11100.13269337668780595No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10850.12970478712276529No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10660.12743345905333436No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10620.12695528472292786No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10490.1254012181491067No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10490.1254012181491067No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT10430.12468395665349696No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCA10420.12456441307089532No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10380.12408623874048882No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10370.12396669515788719No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10010.11966312618422863No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9960.11906540827122049No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9830.11751134169739932No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAACGG9620.11500092646276516No Hit
GGCACAGATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTA9510.11368594705414725No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9390.11225142406292773No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9380.11213188048032612No Hit
GGTTTATTACTGCATGCAAGGTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA9230.1103387267413017No Hit
CCACTCTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAG9090.10866511658487893No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT9070.10842602941967569No Hit
AGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT8990.10746968075886267No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8880.10615470135024475No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8650.10340519895040735No Hit
GTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGCGACGGTCAGGGAGAGTGGAGA8610.10292702462000083No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8590.10268793745479758No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8520.10185113237658619No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8520.10185113237658619No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8510.10173158879398457No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8400.10041660938536667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2450.055.9223251
TACGACA200.00758340651.3571327
TGGTATC2750.051.0519752
GTACGAC306.551929E-445.6507846
GTATCAA18450.042.5138471
GGTATCA7200.039.9611631
TATCAAC21400.036.4823232
GTGTTAT501.9414269E-434.2524261
GCCCGTT400.002694768134.2380877
ATCAACG22600.034.0845533
TCAACGC22900.033.7855154
CAACGCA23250.033.129675
AACGCAG23850.032.5871936
ACGCAGA24650.031.5295987
AGAGTAC25150.031.3111711
GGCGAGG450.004309141531.10904755
CGCAGAG25150.030.9027658
CAGAGTA25550.030.82097810
GTCCACG450.00473608930.51413329
CGATTCA1750.029.39438223
Produced by FastQC (version 0.11.5)