FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281930
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16010.5678714574539779No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGACTCTTG13510.4791969637853367No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10680.37881743695243497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10480.3717234774589437No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAA10190.36143723619338136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9550.3387365658142092No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9460.3355442840421381No Hit
GTCTGGGACTCCAGAGGCCCGATAGGAAAGCGTATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA9180.3256127407512503No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9170.3252580427765757No Hit
GTATAGAGTTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA9140.32419394885255204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8970.31816408328308443No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8960.3178093853084099No Hit
CTCTTGGATAGTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAA8870.3146171035363388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8420.2986556946759834No Hit
ACTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACC8340.2958181108785869No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC8130.28836945341042103No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7190.25502784379101195No Hit
GGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG7060.2504167701202426No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7050.2500620721455681No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACC6590.23374596531053807No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA6440.2284254956904196No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6410.2273614017663959No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGA6230.22097683822225375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6180.21920334834888092No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAGGAAACT6170.21884865037420637No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6150.21813925442485724No Hit
CCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT6050.2145922746781116No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5980.21210938885538963No Hit
GGTCTAGTCAGAGACTCTTGGATAGTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGT5640.20004965771645444No Hit
CTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC5590.19827616784308164No Hit
ATCCAAGAGTCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGT5570.1975667718937325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5490.19472918809633596No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCC5480.1943744901216614No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA5400.19153690632426487No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5380.19082751037491574No Hit
GCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCC5330.18905402050154294No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA5280.1872805306281701No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5160.18302415493207533No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC5040.17876777923598056No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4930.17486610151456036No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4920.17451140353988578No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4870.17273791366651295No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACT4830.1713191217678147No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA4820.17096442379314014No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4780.16954563189444188No Hit
CCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG4760.16883623594509276No Hit
GTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACG4730.16777214202106905No Hit
GGCCAAAAGTGTAAGGAAACTCTATACGTTGCATGCAGTAATAAACTCCA4710.16706274607171992No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4690.1663533501223708No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACTCTCC4690.1663533501223708No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4670.16564395417302166No Hit
GTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT4660.1652892561983471No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4640.16457986024899798No Hit
TTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGA4530.16067818252757776No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4450.15784059873018125No Hit
GTTTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA4420.15677650480615757No Hit
GTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGG4410.156421806831483No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4380.1553577129074593No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTTAGGGAGA4380.1553577129074593No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4340.15393892100876105No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC4320.15322952505941193No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4280.15181073316071367No Hit
GCCCTGGCTTCTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTA4230.15003724328734083No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGA4160.1475543574646189No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4140.14684496151526974No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4030.14294328379384952No Hit
GTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCC3960.14046039797112758No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTATCCAAGAGTCTCTGA3920.13904160607242932No Hit
CACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGC3910.13868690809775475No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG3870.1372681161990565No Hit
GTGTAAGGAAACTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTC3820.13549462632568368No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT3720.13194764657893804No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC3670.1301741567055652No Hit
TATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCT3630.12875536480686695No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC3590.1273365729081687No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT3560.126272478984145No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3550.12591778100947043No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTC3530.1252083850601213No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3520.12485368708544675No Hit
GGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT3490.12378959316142306No Hit
CTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCA3460.12272549923739937No Hit
GGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTAT3450.1223708012627248No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA3440.12201610328805024No Hit
GCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGT3410.12095200936402654No Hit
GTCCAAATAGGTGTATCCATCATCACTATCCAAGAGTCTCTGACTAGACC3410.12095200936402654No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3410.12095200936402654No Hit
GTCTCTGGTGACTCCATCAGCAGTGATAGTTACTACTGGAGCTGGATCCG3400.12059731138935198No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAAT3390.12024261341467742No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT3390.12024261341467742No Hit
GGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGA3370.11953321746532826No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC3330.11811442556663No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3260.11563153974390805No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTTT3250.11527684176923349No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT3250.11527684176923349No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTT3230.11456744581988436No Hit
ACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT3190.1131486539211861No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC3190.1131486539211861No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3180.11279395594651154No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3160.11208455999716241No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT3160.11208455999716241No Hit
TCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACA3150.11172986202248784No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3130.11102046607313872No Hit
GGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATT3100.10995637214911502No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA3100.10995637214911502No Hit
GGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGG3100.10995637214911502No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3090.10960167417444046No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT3090.10960167417444046No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC3080.1092469761997659No Hit
CTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTATCCAAGAGTC3070.10889227822509133No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAGG3060.10853758025041677No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG3020.10711878835171851No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC2960.10499060050367112No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2950.10463590252899656No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT2920.10357180860497286No Hit
TCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCACTGAACCTGTCTGGGACT2900.10286241265562374No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG2900.10286241265562374No Hit
CTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACA2890.10250771468094917No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT2880.10215301670627461No Hit
CTTGGATAGTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT2870.10179831873160004No Hit
GGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAA2870.10179831873160004No Hit
GGCTTCTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTATCCAA2850.10108892278225091No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC2850.10108892278225091No Hit
CCACTGAACCTGTCTGGGACTCCAGAGGCCCGATAGGAAAGCGTATAGATCAGGAGCTGTGGAGACTGCCCTGG2850.10108892278225091No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT2840.10073422480757635No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC2830.10037952683290179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT601.8189894E-1257.451061
CGTCCAT200.006859024552.6684964
CGGAGCC200.0070172852.36563559
TGAAATA200.007203632552.01976847
TGGTATC609.822543E-1151.705952
GGTATCA2550.048.6644251
TACTGAT2250.040.49687270
GTATCAA6600.039.693461
TCGTATG350.001325985639.54939340
TATCAAC7650.034.6959342
GATCAAG400.00253658534.655146
CGTATGC400.002554392734.60571741
CTCGTAT400.002563333634.58107839
CTAGATC400.002588048834.513533
CGTACTA400.00260160934.47675329
CAGCGCT601.3467947E-534.4706344
GCTTGTA501.8671843E-434.4706341
GGCCCAC400.002603874834.4706346
ATCAACG7950.033.8202443
TCAACGC7950.033.8202444
Produced by FastQC (version 0.11.5)