FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281930
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14290.5068634058099528No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGACTCTTG9770.3465399212570496No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9550.3387365658142092No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9450.3351895860674635No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9100.3227751569538538No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAA8710.3089419359415458No Hit
GTCTGGGACTCCAGAGGCCCGATAGGAAAGCGTATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA8700.3085872379668712No Hit
GTATAGAGTTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA8570.30397616429610186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8530.3025573723974036No Hit
ACTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACC8460.30007448657468166No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8340.2958181108785869No Hit
CTCTTGGATAGTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAA8040.28517717163834994No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC7750.27489093037278756No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7700.2731174404994147No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7420.2631858972085269No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7140.2532543539176391No Hit
GGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG6770.24013052885468023No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACC6440.2284254956904196No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6410.2273614017663959No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6370.22594260986769765No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA6200.21991274429823005No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6060.21494697265278614No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAGGAAACT6040.21423757670343702No Hit
CCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT5710.2025325435391764No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5660.20075905366580357No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5610.19898556379243076No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA5360.19011811442556661No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5340.18940871847621749No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5260.18657113467882097No Hit
GGCCAAAAGTGTAAGGAAACTCTATACGTTGCATGCAGTAATAAACTCCA5100.18089596708402794No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5070.17983187316000426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5010.17770368531195688No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4930.17486610151456036No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGA4920.17451140353988578No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA4910.17415670556521123No Hit
GGTCTAGTCAGAGACTCTTGGATAGTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGT4890.17344730961586208No Hit
CTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC4860.1723832156918384No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA4820.17096442379314014No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4770.1691909339197673No Hit
GTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT4760.16883623594509276No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCC4720.1674174440463945No Hit
CCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG4580.1624516724009506No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC4500.15961408860355408No Hit
ATCCAAGAGTCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGT4500.15961408860355408No Hit
GTTTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA4500.15961408860355408No Hit
GCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCC4500.15961408860355408No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4460.15819529670485583No Hit
GGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGA4420.15677650480615757No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4360.15464831695811018No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4300.1525201291100628No Hit
GTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACG4300.1525201291100628No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4260.15110133721136454No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACT4220.14968254531266628No Hit
TTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGA4200.14897314936331715No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACTCTCC4160.1475543574646189No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4100.14542616961657148No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4090.1450714716418969No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4050.14365267974319865No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3990.14152449189515126No Hit
GTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGG3990.14152449189515126No Hit
GGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTAT3930.13939630404710388No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3860.13691341822438194No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC3830.13584932430035823No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTTAGGGAGA3790.13443053240165997No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3770.13372113645231085No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC3690.13088355265491433No Hit
GGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT3570.12662717695881956No Hit
GTGTAAGGAAACTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTC3540.12556308303479588No Hit
GCCCTGGCTTCTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTA3510.12449898911077219No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTC3510.12449898911077219No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA3490.12378959316142306No Hit
GTGCCTGACCCACTGCCACTGAACCTGTCTGGGACTCCAGAGGCCCGATA3470.12308019721207393No Hit
CTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCA3370.11953321746532826No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3360.1191785194906537No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC3360.1191785194906537No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA3350.11882382151597913No Hit
CACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGC3320.11775972759195544No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGA3280.11634093569325718No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3260.11563153974390805No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA3240.11492214379455892No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG3230.11456744581988436No Hit
GTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCC3220.1142127478452098No Hit
GTCCAAATAGGTGTATCCATCATCACTATCCAAGAGTCTCTGACTAGACC3190.1131486539211861No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTATCCAAGAGTCTCTGA3180.11279395594651154No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTTT3110.11031107012378959No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3100.10995637214911502No Hit
GTCTCTGGTGACTCCATCAGCAGTGATAGTTACTACTGGAGCTGGATCCG3100.10995637214911502No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3090.10960167417444046No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT3070.10889227822509133No Hit
GGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAA3050.1081828822757422No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAAT3030.10747348632639307No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT3020.10711878835171851No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2930.10392650657964743No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC2900.10286241265562374No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC2890.10250771468094917No Hit
GCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGT2880.10215301670627461No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT2870.10179831873160004No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAGG2870.10179831873160004No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC2870.10179831873160004No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT2860.10144362075692548No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTT2860.10144362075692548No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT2860.10144362075692548No Hit
TATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCT2840.10073422480757635No Hit
TTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAG2820.10002482885822722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT150.002386323468.76086413
GAAGCAT150.002386323468.7608645
GTGGTAT1000.065.3576051
TGGTATC1000.065.334412
GTTGTCT200.007350876551.75427231
TGTTGTC200.00736123351.7358530
GCGATAT200.007397566551.6714826
GCTTGAT200.007439253451.5981061
GTATCAA5550.040.906611
TAGCAAT350.001354984639.37574818
TATCAAC6200.036.0503962
ATCAACG6250.035.7493063
TCAACGC6300.035.4655844
AACGCAG6550.034.6551746
CAACGCA6450.034.6469465
GGGGGAT601.3513045E-534.45377718
ACGGGAT400.00261695534.43541316
AGAGCAT2153.092282E-1134.191370
GGTATCA2150.033.5749323
TGTTAGA2700.032.17666270
Produced by FastQC (version 0.11.5)