FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577066
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23640.40965851393081554No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC14340.24849843865346424No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC7570.1311808354676935No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT7200.12476909053730423No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG6840.11853063601043902No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG6240.108133211798997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.043.2610441
GTGGTAT6050.042.0454441
GTATCAA49300.039.0763931
TGGTATC6850.036.614482
TATCAAC59700.032.019642
ATCAACG61850.030.778313
TCAACGC61900.030.7013914
AACGCAG62950.030.30486
CAACGCA62850.030.2991055
GTCGGTT1003.0880437E-629.75129369
CGCAGAG65750.029.0167688
TCGGATA500.006244061528.83463164
ACGCAGA67350.028.2771177
GCAGAGT67250.028.2687749
CATCTAG500.007314134427.91569342
AGAGTAC67750.027.76004211
CAGAGTA68050.027.6376610
CCGAATT1504.3655746E-1127.10275714
ACGGGTC1900.026.77631216
CGAATTA1405.7661964E-1026.62108615
Produced by FastQC (version 0.11.5)