FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588593
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT71420.4495802260239092No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAAAACACT57550.3622702605387283No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55360.34848447651475234No Hit
GTATTATAATACTCCAGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT53680.3379090805511544No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTCCAGCCTCAGGGACA52970.3334397167808243No Hit
GTCTATTACTGTGCGAGACAGTTGCGTTTGTATGAGAGTACTTCTTATCT51780.325948811306609No Hit
GTCTCTGGTGGCTCCATCAACACTACTAGTTACTACTGGGGCTGGATCCG44500.28012209546435113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41840.2633777185219877No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37860.23832410189394013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35230.22176857130806948No Hit
GTTTTATTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAGAA34460.21692151482475375No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34040.21427766583385424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33170.20880112149556243No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA31290.19696674982201232No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30940.19476354232959606No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28950.18223673401557228No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC27940.17587890668031397No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27700.17436813582837138No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATT27520.17323505768941447No Hit
GTTTATTACTGTCAGCAGTATTATAATACTCCAGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA26990.1698987720580413No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26330.16574415221519923No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26270.1653664595022136No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25570.1609600445173811No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24750.15579824410657733No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24370.15340619025766827No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC24340.15321734390117545No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAA23480.14780374834838125No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23410.14736310684989798No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22950.14446746271700808No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22220.13987220137568276No Hit
TTATAATACTGCTGACAGTAATAAACTGCTACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22160.1394945086626971No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22120.1392427135207067No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAACAATAAGAACTA22070.13892796959321865No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC21820.1373542499557785No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21740.13685065967179763No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGTA21620.13609527424582635No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21560.1357175815328407No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21160.1331996301129364No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20580.12954860055407522No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20410.1284784712006159No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20310.12784898334563982No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA19950.12558282706772597No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19340.12174295115237194No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTAGCAGTTTATTACTGTCAGC19010.11966564123095091No Hit
GGCCAAAAGTGTCTGGAGTATTATAATACTGCTGACAGTAATAAACTGCT18910.11903615337597484No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18690.11765128009502748No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18440.1160775604575873No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18430.11601461167208969No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTAGCAGTTTATTACTGTCAGCAGTATTATAATACT18110.11400025053616629No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17970.11311896753919978No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17970.11311896753919978No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17590.11072691369029071No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17390.10946793798033856No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16910.10644639627645344No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16730.10531331813749652No Hit
GAATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16650.10480972785351565No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16430.1034248545725683No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA16420.10336190578707069No Hit
AGGCTGGACTCTGTGACCGCCGCAGACACGGCTGTCTATTACTGTGCGAG16330.10279536671759225No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16220.10210293007711857No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16220.10210293007711857No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16060.10109574950915685No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15890.10002562015569752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.051.2181551
GGTATCA15450.044.7692681
TGGTATC5150.043.413732
GTATCAA38500.040.579581
TACGGAT17600.040.16500570
GTCTATA655.562906E-737.0592881
TCGTATG1051.6370905E-1136.22542640
CGTATGC1003.7107384E-1034.59414341
TATCAAC46100.034.0985762
ATCAACG49000.032.1506963
TCAACGC49800.031.6342164
CAACGCA50750.031.1776055
AACGCAG51400.030.7833376
ACGAGAC1251.2914825E-1030.27224522
CTCGTAT1151.6698323E-930.05711739
TGTTAGA12100.028.66997170
CGCAGAG55100.028.650188
GCAGAGT57950.027.3005079
CTACGGA17750.027.06408969
CAGAGTA58150.026.85173810
Produced by FastQC (version 0.11.5)