FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822513
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91671.1145112600044011No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC21420.26042141583172546No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC9570.11635074460829191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA150.00270986766.602166
TAACGTT150.00270986766.602165
CTAACGT150.002710520366.598114
ACTTATG200.006024808654.43309455
TAACCTT400.00639057453.62299370
ACGTTAG200.00845839649.957697
ATATGAT200.008462463549.9516145
AGTTAAG200.008462463549.9516146
GTGGTAT12250.047.61061
ATAGTAC307.507073E-444.40683411
TTAGATC957.2759576E-1238.554453
AATCATA400.001591679838.1146667
AGCTATA350.001599583738.0768972
GTATCAA93750.037.7177541
AATTGGC400.002048819636.20476554
GGTATCA42800.034.8847771
TATCAAC103150.034.2701262
AGATTAC400.003085520633.30512611
AGGTATC1950.032.4629942
TGGTATC18450.031.962922
Produced by FastQC (version 0.11.5)