FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176799
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCA55070.46796436774674355No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53940.45836204823423543No Hit
GTATTACTGTCAGCAATATGTTGATATTCCCCTCACTTTCGGCGGAGGGA52710.4479099659330098No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42870.3642933075232049No Hit
GTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCA42370.36004449357961726No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGATGTATAAAAGACT36420.30948360765092425No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36100.30676436672702817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36000.3059146039383106No Hit
GTGTATTACTGTCAGCAATATGTTGATATTCCCCTCACTTTCGGCGGAGGGACCAGGGCGGAGATCAAACGAA35870.3048099123129778No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35330.3002211932539032No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33740.2867099649132945No Hit
CTTTTATACATCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA32830.27897712353596493No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32260.274133475640275No Hit
CTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGTGGGTCTG30800.2617269389249991No Hit
GTGCAGCCACAGTTCGTTTGATCTCCGCCCTGGTCCCTCCGCCGAAAGTG30330.2577330538180267No Hit
GTCCCTGACCGATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCAC29810.25331428731669553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29790.25314433475895204No Hit
ATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGCC29370.2495753310463384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28050.238358462235267No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26750.22731154598193914No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25710.21847401297927682No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25580.21736932135394404No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAA25260.21465008043004796No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA24620.20921159858225574No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23480.19952430279087593No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22770.19349098699098147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22380.1901769121149831No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCGCCCTGGTCC22150.1882224577009328No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22010.18703278979672824No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21910.18618302700801073No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCACTGAATCGGTCAGGGACC21910.18618302700801073No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20790.1766656837743744No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA20460.17386146657160653No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19590.16646853030976402No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19040.16179483497181762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18920.1607751196253566No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGATG18840.16009530939438257No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTGTATTACTGTCAGCAATATGTTGATATTCCCCTCACTTT18570.15780094986484522No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18530.1574610447493582No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACATCTCCAA18220.15482678010433387No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17930.15236246801705305No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17910.15219251545930954No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACATCTCCAACAATAAGAACTA17520.14887844058331115No Hit
GTAATAGACGGCCGTGTCTGCGGCGCTCACAGACCTCAGTTTCAGGGACA17520.14887844058331115No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17440.14819863035233713No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17240.14649910477490208No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16890.14352493501439073No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16730.1421653145524427No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16440.13970100246516187No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15810.1343474968962414No Hit
GACCAGGGCGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15800.13426252061736968No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15640.1329029001554216No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15280.12984375411603852No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15060.12797427598085995No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGC15040.12780432342311643No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14910.12669963179778365No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAAT14850.12618977412455312No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14570.12381043831614406No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATT14550.12364048575840056No Hit
ATGTTGATATTCCCCTCACTTTCGGCGGAGGGACCAGGGCGGAGATCAAA14400.12236584157532425No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14120.11998650576691516No Hit
GCTGATGGTGAGAGTGAAATCTGTCGCAGACCCACTGCCACTGAATCGGT14110.11990152948804342No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14010.11905176669932589No Hit
GGCTGAAGATGTGGCAGTGTATTACTGTCAGCAATATGTTGATATTCCCC13960.11862688530496711No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13870.11786209879512134No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13850.11769214623737784No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATC13740.11675740716978855No Hit
GAATATCAACATATTGCTGACAGTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13720.11658745461204505No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13690.1163325257754298No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13550.11514285787122525No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13510.11480295275573824No Hit
GACCGATTCAGTGGCAGTGGGTCTGCGACAGATTTCACTCTCACCATCAG13290.11293347462055968No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13190.11208371183184214No Hit
GTCTGCGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12990.11038418625440709No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12950.11004428113892006No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12950.11004428113892006No Hit
GCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGC12850.10919451835020254No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12540.10656025370517822No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12390.1052856095221019No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12260.10418091789676913No Hit
GCCCTGGTCCCTCCGCCGAAAGTGAGGGGAATATCAACATATTGCTGACAGTAATACACTGCCACATCTTCAGC12100.10282129743482107No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11870.10086684302077074No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGA11830.10052693790528373No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11830.10052693790528373No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11810.10035698534754023No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11800.10027200906866848No Hit
TTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGTGGGTCTGCGACAGATTTCACT11770.10001708023205322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)