FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332945
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTACACCTCGCACAGTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGTTCCAGGGAGAACTGGCT19220.14419199591881135No Hit
GTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGTTCCAGGGAGAACTGGCTCTCGGACGCGTCTAGTGA18490.13871540086050063No Hit
GTCTCTAGTGACTCCATCACTGACTCCATCAGTCGTTACTACTGGAGCTGGATCCGGCAGGCCCCAGGGAAGGAA15620.1171841298778269No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15040.11283286257122385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14760.1107322507680362No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14480.10863163896484851No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13620.10217975985505778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)