FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332945
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28950.21718825607958317No Hit
GTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGTTCCAGGGAGAACTGGCTCTCGGACGCGTCTAGTGA16770.12581164264091918No Hit
CCACTACACCTCGCACAGTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGTTCCAGGGAGAACTGGCT16760.12573662079080533No Hit
GTCTCTAGTGACTCCATCACTGACTCCATCAGTCGTTACTACTGGAGCTGGATCCGGCAGGCCCCAGGGAAGGAA14910.11185757851974387No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14380.10788142046371006No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13460.10097941025323627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31600.039.5205571
GGTATCA13450.038.0077971
TATCAAC35100.034.9762042
ATCAACG35950.033.8528983
TCAACGC36150.033.6656074
CAACGCA36950.032.941665
AACGCAG37500.032.552396
ACGCAGA39350.030.7628637
AGAGTAC41300.029.64250811
CGCAGAG41450.029.2870438
GTGGTAT7250.027.9204711
GCAGAGT43800.027.872299
CAGAGTA43950.027.8551910
AGTACGG32100.026.81122213
GAGTACG32950.026.22462712
TGGTATC7250.025.5418622
CGCGTCT8550.024.42261164
GCGTCTA8350.024.2377965
CGTCTAG8700.023.45685266
TCGGACG8850.023.40885559
Produced by FastQC (version 0.11.5)