FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923443
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT34580.3744681588360083No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31750.3438219792667225No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACCTAAGTAGTTCTTATTGTTGGAGCTGTAAAAAACACT30920.33483387713156093No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21910.23726423829083118No Hit
GTACTATAATAATGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT21350.23119997660927638No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20790.22513571492772158No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20560.22264503602279728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20460.22156213215109108No Hit
TTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT20410.221020680215238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19840.21484812814651258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19410.21019164149817582No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19080.20661805872154532No Hit
TTATTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT17520.18972475832292843No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17450.18896672561273406No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC17410.1885335640640516No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16270.17618845992660076No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16220.17564700799074767No Hit
CTATAATAATGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC16210.17553871760357706No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16050.1738060714088471No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14780.1600531922381782No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14570.15777909410759516No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14430.15626302868720646No Hit
ATTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAA13690.14824954003658047No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13690.14824954003658047No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13620.14749150732638613No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13610.14738321693921552No Hit
GTTTATTACTGTCAGCATTATTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA13480.14597544190599743No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT13290.14391792454975563No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC13120.1420769879678551No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13040.1412106648704901No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12900.13969459945010143No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12710.1376370820938596No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT12320.13341375699420538No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12080.13081478770211047No Hit
GTGTATTACTGTGCGAGAGATGCCCAAGGATACTGGGGTGCCCTTGACTA12040.130381626153428No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA12040.130381626153428No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTA11720.1269163337639681No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC11710.1268080433767975No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11630.1259417202794325No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11620.1258334298922619No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11520.12475052602055568No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11330.12269300866431389No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11030.11944429704919524No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT10990.11901113550051275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10750.11641216620841785No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10500.1137049065291523No Hit
ACTTAGGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATT10410.11273029304461674No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10410.11273029304461674No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCATTATTATAGTACT10360.1121888411087636No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10330.11186396994725176No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9790.10601628904003821No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCATTATTATAGTACTCCTC9780.1059079986528676No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCATTATTATAGTACTCCTCCGACTTT9750.1055831274913557No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG9720.10525825632984386No Hit
GTCCTGGTTTCTGCTGGTACCAACCTAAGTAGTTCTTATTGTTGGAGCTG9490.10276757742491956No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG9380.10157638316604274No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9370.1014680927788721No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9350.10125151200453086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2450.050.4396481
GGTATCA8100.038.988671
TGGTATC3350.036.8667142
GTATCAA18750.036.2492941
TATCAAC20700.032.8149382
TCAACGC21450.031.6607024
ATCAACG21750.031.2240033
CCTTTGT7050.031.22038870
CAACGCA22150.030.6618025
AACGCAG22150.030.5069456
ACGCAGA23300.029.0028067
AGAGTAC23900.028.70528211
CGCAGAG24050.028.5262478
AAACGTG500.00790945827.473518
CAGAGTA25200.027.36057510
GCAGAGT25250.027.3063969
TCACGAA658.851762E-426.43689727
GCGTTGT7100.025.78520462
GTATTGG809.892173E-525.7452371
CGTTGTC7150.025.6490963
Produced by FastQC (version 0.11.5)