FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289706
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACAGCTCCAACAAATACAACTACTTAGCTTGGTACCAGCAGAG159311.235242760753226No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63310.49088707038658425No Hit
GTATTATCGTAGTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT61080.47359630799577573No Hit
GACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCGACCCGGGAA53520.4149782973794027No Hit
GTATAGAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA53030.41117898187648966No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCGACCCGGGAATCCGGGGTCCCT51410.39861797960155265No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49710.38543668091797667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44210.34279130282405446No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTGTATTTGTTGGAGCTGTATAGAACACT42350.3283694113232008No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCC42260.32767157786348206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41580.3223990583900517No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37750.29270236782646586No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCAT35850.27797032812129274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34430.26696006686795287No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34380.2665723816125536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33380.2588186765045677No Hit
GTTTATTACTGTCAGCAGTATTATCGTAGTCCGACGTTCGGCCAAGGGAC30090.23330898669929429No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28630.22198857724163493No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTGTATTTGTTGGAGCTG28190.21857694699412117No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTCTATA28150.2182667987898017No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28090.21780157648332255No Hit
GTCCCTGAGCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC26320.2040775184421876No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC25030.19407523885288583No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24780.19213681257588938No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24330.18864764527729574No Hit
GCTCATTTACTGGGCATCGACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCAGCGGGTCTGAGACAGATTT24080.18670921900029927No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23410.18151423657794877No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATCGTAGTCCGACGTTCGG23210.1799634955563516No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23080.17895551389231346No Hit
CGATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22930.17779245812611558No Hit
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA22540.17476851313400107No Hit
GTCTGAGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG22450.17407067967428236No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG21940.17011629006920959No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTCTATACAGCTCCAA21820.16918584545625126No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21620.1676351044346541No Hit
ACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGC21520.16685973392385553No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTCTATACAGCTCCAACAAATACAACTA21180.16422347418714034No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGC21160.1640684000849806No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20290.15732267664103292No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20200.1566248431813142No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20070.15561686151727602No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20000.15507410215971704No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTA19950.15468641690431773No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19320.14980158268628666No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATCGTAGT19240.1491812862776478No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19150.14848345281792905No Hit
GTGTATTACTGTGCGCGAGTGTTCCCGTATAGCAGCAGCTGGTACGGTCC19090.1480182305114499No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19060.14778561935821033No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18980.14716532294957146No Hit
CAGTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATA18940.14685517474525203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18840.14607980423445344No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATCGTAGTCCGA18810.14584719308121386No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA18460.1431333962934188No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18460.1431333962934188No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18260.14158265527182165No Hit
ATACAGCTCCAACAAATACAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTA18100.14034206245454392No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17730.13747319156458915No Hit
CTGCTGGTACCAAGCTAAGTAGTTGTATTTGTTGGAGCTGTATAGAACAC17380.1347593947767941No Hit
ATCGTAGTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACT17330.1343717095213948No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16940.13134776452928032No Hit
CCACTGAATCGCTCAGGGACCCCGGATTCCCGGGTCGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGT16890.13096007927388104No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16630.12894411594580468No Hit
CATTTACTGGGCATCGACCCGGGAATCCGGGGTCCCTGAGCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCA15880.12312883711481531No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15820.12266361480833617No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15770.12227592955293687No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15580.12080272558241956No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15560.12064765148025985No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15340.11894183635650295No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15320.11878676225434324No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15320.11878676225434324No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTG15250.11824400289678423No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15140.1173910953349058No Hit
GAGCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAG14950.11591789136438849No Hit
GCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCGACCCGGGAATCCGGGGTCCCTGAGCGATT14920.11568528021114889No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14810.11483237264927044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.11335916867875313No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT14360.11134320535067682No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14340.1111881312485171No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14150.1097149272779998No Hit
TTATTACTGTCAGCAGTATTATCGTAGTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG14010.10862940856288177No Hit
CTCAGGGACCCCGGATTCCCGGGTCGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTCTCTGCTGGTA13950.10816418625640262No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13950.10816418625640262No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13830.10723374164344432No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13810.10707866754128462No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13610.10552792651968744No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13540.10498516716212843No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA13520.1048300930599687No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13440.10420979665132983No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13420.10405472254917013No Hit
ACGATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13360.10358950024269098No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13270.10289166678297225No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13270.10289166678297225No Hit
GTCCTGTGCAGCCTCTGGATTCATCTTTAATACCTATTGGATGAGCTGGGTCCGCCAGACTCCAGGGAAGGGG13020.10095324050597577No Hit
TTCCCGGGTCGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTC12960.10048801819949663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA208.637063E-568.8012544
ATCTCGA200.007279518551.89273540
GTGGTAT4250.050.201971
TGGTATC4450.048.7020152
GTATCAA35400.042.772771
GGTATCA12800.041.6715551
CGGATCC350.001364522339.33025724
CGTATGC1250.035.99580841
TATCAAC41900.035.9605642
ATCAACG43350.034.916443
TCAACGC43700.034.7155084
CAACGCA45350.033.604145
AACGCAG45850.033.2376866
ATCTCCG1550.031.0679210
CGCAGAG49550.030.8886228
GCTTGTA1800.030.589011
GTCTATA450.00468858730.5783392
ATCTCGT1251.2369128E-1030.39750537
AGAGTAC50400.030.37003911
GCAGAGT50850.030.303039
Produced by FastQC (version 0.11.5)