FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561044
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148372.644534118536158No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC16810.29961999415375623No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC13000.23171088185596853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.1390265291135811No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC7750.1381353334141351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.13742237685457825No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG6780.12084613684488203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.11371657124931377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.11318185382964616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.10551757081441028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG150.002969737965.0783115
AGTGTAC150.00297078865.072510
CTTGTAT1450.051.64607216
ACTGATT250.00787144250.8708868
GTATCTT250.00787144250.8708868
GTGGTAT8500.049.004511
GTGTACC200.00927467748.8043811
TTGTATC1550.048.3313217
TTGTAAG305.393632E-447.48522644
TACCACT1900.046.235738
GGCTAAG356.8093045E-445.29083666
TTACCGT657.594433E-444.29081770
TCTAGAG700.001093736341.1271970
TAAGGCT603.1230775E-739.8832338
ATTCGAG750.00153540538.38537670
GCGTCTA704.5928937E-737.96742660
GTATCAA78050.037.3161
TGTATCA2100.035.68586318
TACATCT658.3120176E-735.1991425
GGTATCA36650.033.740741
Produced by FastQC (version 0.11.5)