FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756425
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104571.3824239019069968No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC19880.26281521631358035No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC9350.12360776018772515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.11805532604025515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.11580791221866013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.10232342928909012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTACT200.00882605849.42229516
GTGGTAT12200.046.4361841
CATACGA307.9286814E-443.9163931
GTATCAA92150.038.173661
ACGTTCT1001.3096724E-1038.0898964
TACGCTA802.9649527E-838.0898964
TATCAAC103850.033.7465062
GATTATA450.003077393233.3221261
GGTATCA42600.033.014611
TACCACG502.4478373E-432.9263957
TAGGTAC400.003274842932.902453
ATACTAA450.003640178832.19917350
ATCAACG114950.030.4837843
TCAACGC114650.030.3666784
GTACGCT1002.2202948E-730.29108263
AACGCAG115700.029.9951786
CAACGCA116150.029.986415
TGGCCTA950.00574843729.32417170
AAGCGTA953.0833144E-729.17718340
TGGTATC19850.028.679482
Produced by FastQC (version 0.11.5)