FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141548
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGA33660.2948627652976485No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26950.2360829329997512No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25300.22162887587731747No Hit
CCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCT23970.2099780298331739No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21690.19000515090035636No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT21260.18623833601390394No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC21250.18615073566770737No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20920.1832599242432206No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT20020.17537589308552948No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC19920.1744998896235638No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATACATTGCCGAAG19870.17406188789258095No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18770.16442584981095845No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18560.1625862425408305No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18480.16188543977125797No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG17870.15654181865326733No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC17550.15373860757497715No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17490.15321300549779773No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17410.1525122027282252No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC16990.14883298818796933No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGT16840.1475189829950208No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16810.1472561819564311No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC16600.14541657468630315No Hit
GACTATCACTGTCAATCAGCGGACACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT16390.1435769674161752No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATAC15960.1398101525297228No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATA15870.13902174941395368No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA15700.13753254352861202No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15500.13578053660468067No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14810.12973611271711746No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14790.12956091202472433No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14760.12929811098613464No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA13920.12193968190562289No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13390.11729686355720478No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12730.11151524070823128No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGG12720.11142764036203472No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC12360.10827402789895825No Hit
GTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGA12300.10774842582177883No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12260.10739802443699258No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12170.10660962132122347No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA11970.10485761439729209No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG11960.10477001405109553No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCACATAGACTGTGTTGATGGACGTGTCCCTGGT11610.10170400193421564No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTCGGCAATGTATCTCCAGAGCAGGTGATCCTGGC11610.10170400193421564No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTC11550.10117839985703622No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11550.10117839985703622No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11420.10003959535648085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3350.053.3438031
ATATCGC200.007384347351.70503234
TGGTATC3950.044.3495332
GGTATCA10450.039.7918851
TACCGTC451.0288015E-438.1590357
GTATCAA21750.035.866751
ACGTCAC501.8378127E-434.5749641
CGCTAGT450.004325620431.08578156
ATCAACG26000.029.8390253
TCAACGC26400.029.2594554
CAACGCA27000.028.6142585
AACGCAG27200.028.5338486
CGCAGAG29450.026.4716118
AGAGTAC29600.026.22144311
CAGAGTA30200.025.70048710
GCAGAGT30900.025.229429
ACGCAGA31400.024.8276777
AGTACGG25650.023.963413
GAGTACG26400.023.54585812
TTCGGGG5950.021.71454870
Produced by FastQC (version 0.11.5)