FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868528
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTTTTTCCTGGTCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT73650.39416053706446996No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64300.3441211477697953No Hit
GTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC60900.3259250062080954No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41380.22145774641857122No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39860.21332300077922298No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39480.21128931436938594No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38940.20839933894488066No Hit
GAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATAAGGCGT38740.2073289776765454No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36780.19683943724685954No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35640.19073837801734841No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA34880.18667100519767432No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT34300.183566957519502No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGAC31170.16681580367005472No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30620.16387231018213266No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29750.15921623866487417No Hit
GTCCTGATCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATT29380.15723607031845388No Hit
GATCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA29180.1561657090501186No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28340.1516701917231104No Hit
CCTTCCACCCTGTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCG27840.14899428855227217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27830.1489407704888554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27650.14797744534735363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27500.14717467439610218No Hit
TCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT27470.14701412020585186No Hit
GTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA25350.13566829076149783No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25310.13545421850783076No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23720.12694484642456522No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC23530.1259280032196467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23520.12587448515622993No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23450.12549985871231256No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22640.12116489557555465No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22510.12046916075113673No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22300.11934528141938466No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21810.11672289631196323No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21700.11613419761437879No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21680.11602716148754527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21180.11335125831670706No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20960.11217386092153823No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19960.1068220545798618No Hit
ATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGA19710.10548410299444269No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19500.10436022366269063No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA19380.10371800690168946No Hit
ACTTATTACTGCCAACAGTATAGTACTTTTTCCTGGTCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA19210.10280819982360445No Hit
CCTTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTACTAATACTCTGA18900.10114913985768477No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18760.10039988696985006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA503.0946295E-453.14956770
GTGGTAT10200.041.323031
GTATCAA77600.039.7878341
TATCAAC89800.034.2086372
TCAACGC93950.032.8240284
ATCAACG94000.032.77633
CAACGCA95750.032.2112855
AACGCAG96250.032.1497846
ACGCAGA102000.030.337427
CGCAGAG104150.029.7111558
ACGGGTA4450.029.24756816
AGAGTAC105500.029.16873611
GCAGAGT108250.028.5542149
AGTACGG78700.024.96458213
GAGTACG79300.024.8195212
GAGTACT46450.023.72842612
GGTATCA65300.023.5624471
TGTTAGA14100.023.55920670
GCTTGTA5100.023.5171721
TACGGGT11700.023.11707915
Produced by FastQC (version 0.11.5)