FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338993
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT29040.8566548571799417No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC7980.23540309091928152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.2002991212207922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.16136026407624937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.13009118182381346No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC4220.12448634632573534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTATC150.002960489665.1239313
CATGATA200.00437043959.02255266
AGGGGGG5850.058.51978770
TGAACTA200.006390232653.6212444
GCGGTAT253.4020314E-452.129911
TATCGTA750.050.1395138
TGATACT250.00836793650.08434368
GGCTTAT200.00853109549.84304432
CTTATAC6100.049.1388471
AATGTGC200.00924264248.8429516
GCTCTTA200.00924803248.8357477
CTTGAAA6550.047.134557
GCTTGAA6750.046.20899256
GTCTTCT6500.046.18909549
TTATACA6600.045.8827672
TATACAC6600.045.8827673
TCGTATG6650.045.32839240
ATCGTAT1000.044.73285339
TCGCAAA307.265126E-444.6952234
GTGGTAT5850.044.5554731
Produced by FastQC (version 0.11.5)