FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629164
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGACCCCAGCCTCAGGGAGA57830.354967332938857No Hit
GTATATTACTGTGCACCCGGTACAGCAGAAGCTGGTGGGGGATTGGGGTGGTTCGACCCCTGGGGCCAGGGAAGC42310.259703749898721No Hit
GTATAATAACTGGTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT42180.2589057946284106No Hit
GTCTCTGGTGGCTCCATCAGCAGTACCATTTACTACTGGAGCTGGATCCG39900.24491088681065873No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35420.21741212057226894No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT35150.2157548288570089No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34760.21336096304607763No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32170.19746323881450856No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29460.1808289404872683No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28720.17628673356396288No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27210.16701817619343418No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26390.16198491987301464No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25460.15627647063156316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25320.15541713418661351No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACCATTTACTACTGGA25000.1534529365981571No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23670.14528924037113514No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21890.13436339128534636No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT21490.13190814429977585No Hit
CTGGTACCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCACT21280.13061913963235133No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20950.12859356086925564No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20740.12730455620183112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20700.12705903150327408No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20680.12693626915399556No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20610.12650660093152072No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20590.12638383858224217No Hit
GTACAGCAGAAGCTGGTGGGGGATTGGGGTGGTTCGACCCCTGGGGCCAGGGAAGCCTGGTCACCGTCTCCTCAG20490.12577002683584954No Hit
GTCCAGGGATATGGTGACTCGACTCTTGAGAGACGGGTTGTAATATGTGGTCCCACTAAAATAGATACTCCCAAT19930.12233268105605083No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19750.1212278199125441No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT19690.12085953286470853No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19440.11932500349872695No Hit
TCCCTGGACACCTCCAAGAACCACTTCTCCCTGAGGCTGGGGTCTGTGACCGCCGCAGACACGGCTGTATATTA19040.11686975651315644No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18800.11539660832181413No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18580.11404622247975035No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18490.11349379190799698No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18470.11337102955871844No Hit
GGTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC18340.11257307428840803No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG18140.11134545079562279No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT18120.11122268844634425No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17810.1093198720325271No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17590.10796948619046333No Hit
TCCCTGACCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCA17550.10772396149190627No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17530.10760119914262774No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA17270.10600528860200693No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA17250.1058825262527284No Hit
GGACTGGTGAGGCCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACCATTTA17050.10465490275994313No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17020.10447075923602535No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTTAGCCTGGTA16300.10005131466199842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28600.039.0802731
ATATCGT459.972518E-538.3626337
TATACAC5600.037.439393
CAGGGAT21950.037.31132570
CTTATAC5500.036.8917771
CTAGCGC5350.036.66639328
CGTATGC4500.036.14668341
GGTATCA12100.035.527521
TAGCGCT5350.035.38855429
ACTAGCG5450.035.3556327
TCGTATG4050.035.01198640
TATTCGG400.002467916334.85954353
TATCAAC31900.034.8015632
CGAGACT5650.034.68858723
ATTACGT400.00264534634.3695317
TTATACA6050.033.518422
ATCAACG33850.032.796753
CTCGTAT3300.032.472939
AGACTAG6050.032.40308825
TATGCCG5050.032.23880843
Produced by FastQC (version 0.11.5)