FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629164
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGACCCCAGCCTCAGGGAGA55770.3423228109631688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49270.302425047447648No Hit
GTATATTACTGTGCACCCGGTACAGCAGAAGCTGGTGGGGGATTGGGGTGGTTCGACCCCTGGGGCCAGGGAAGC39870.24472674328674093No Hit
GTATAATAACTGGTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT39850.24460398093746238No Hit
GTCTCTGGTGGCTCCATCAGCAGTACCATTTACTACTGGAGCTGGATCCG37020.22723310851455103No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT34720.21311543834752056No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34260.21029190431411446No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32810.20139163399142138No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30190.1853097662359345No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28090.1724197195616893No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26470.16247596927012872No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23140.1420360381152542No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23080.1416677510674186No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACCATTTACTACTGGA23070.14160636989277936No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22750.13964217230432296No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT21840.13405648541215004No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21270.13055775845771206No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20630.12662936328079924No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20450.1255245021372925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20360.12497207156553915No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19580.12018433994367664No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19570.12012295876903736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19490.11963190937192326No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGGTACCAGTGGCCCTGGA19300.11846566705377727No Hit
GTCCAGGGATATGGTGACTCGACTCTTGAGAGACGGGTTGTAATATGTGGTCCCACTAAAATAGATACTCCCAAT19240.11809738000594171No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19090.11717666238635276No Hit
CTGGTACCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGAGTTCACT18890.11594903889356749No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18620.1142917471783074No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17790.10919710968324858No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17740.10889020381005227No Hit
TCCCTGGACACCTCCAAGAACCACTTCTCCCTGAGGCTGGGGTCTGTGACCGCCGCAGACACGGCTGTATATTA17650.1083377732382989No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17510.10747843679334923No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17490.10735567444407071No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17110.10502318980777871No Hit
GGTCAGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC17110.10502318980777871No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17050.10465490275994313No Hit
CTGCTGTACCGGGTGCACAGTAATATACAGCCGTGTCTGCGGCGGTCACA17020.10447075923602535No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17010.10440937806138609No Hit
GTACAGCAGAAGCTGGTGGGGGATTGGGGTGGTTCGACCCCTGGGGCCAGGGAAGCCTGGTCACCGTCTCCTCAG16930.10391832866427199No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16890.10367280396571493No Hit
TCCCTGACCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCA16680.10238379929829042No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16410.10072650758303033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.039.615921
GTATCAA32600.037.817911
CAGGGAT23650.033.5928970
TATCAAC36850.033.344642
ATCAACG39350.031.2089163
TCAACGC39550.031.135924
CAACGCA40250.030.512955
AACGCAG41100.029.8819056
CGCAGAG42800.028.6958878
TGGTATC8400.028.6021882
GTGGTAT8300.028.549051
AGAGTAC43850.028.00875711
GCAGAGT45250.027.2180069
TGTTAGA8300.026.63499670
CAGAGTA47100.026.14893210
ACGCAGA47500.025.784287
AGTACGG38300.024.5417513
GAGTACG38700.024.20093212
TACGGGA13150.023.74448415
ACGGGAT2900.023.67368516
Produced by FastQC (version 0.11.5)