FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304259
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76390.3315165526097544No Hit
GTAGTATATGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGGAGAAACA65000.282086345328368No Hit
GGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTGATCGGGCC57600.2499718998602154No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55340.2401639746226444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52780.22905411240663484No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51240.2223708359173166No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46690.20262479174433082No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45010.19533394466507456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44880.1947697719744178No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG43530.188911055571444No Hit
GATTTTACACTGACAGTCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCATCAAATT43140.18721853749947379No Hit
GTACCTGCAGAGGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTGATCGGGCCTCCGGGGTCCCTGA42440.18418068454978367No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT42160.18296554336990764No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41970.18214098328356318No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA41500.18010128201734266No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGATTTTACACTGAC40890.17745401016118414No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39610.17189907905317936No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATGGATACAAGTATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTC39300.17055374417545946No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT38070.1652158025638611No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37620.16326289709620315No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37250.16165717482279554No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36960.1603986357436382No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36180.15701359959969777No Hit
GGCCAAAAGTGTACGGAATTTGATGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGA35570.15436632774353923No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35130.1524568201751626No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGACTGTCAGTGTAA34840.15119828109600525No Hit
ATATACTACTGTGCGAAAGTGCAGTTTTTGGAGTCGTTTTTCCAGTCACAATATTACTACTATACTATGGACGT34160.14824722394487774No Hit
GTATAGTAGTAATATTGTGACTGGAAAAACGACTCCAAAAACTGCACTTT34050.14776984705278357No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34010.1475962554556584No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA33940.1472924701606894No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33750.14646791007434493No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC33750.14646791007434493No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC33280.14442820880812443No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG33270.14438481090884314No Hit
GTGTAAAATCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG32830.1424753033404665No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32530.14117336636202787No Hit
GTATATGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGGAGAAACAGCG32050.1390902671965261No Hit
GCCCTGGCCTCTGCAGGTACCAATCCAAATACTTGTATCCATTACTATGG29980.1301069020452996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29550.12824079237620423No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28660.12437837934016965No Hit
CTACTATACTATGGACGTCTGGGGCCAAGGGACCACGGTCAGCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGT28420.12333682975741876No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28050.12173110748401113No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA27990.12147072008832341No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27460.11917063142641517No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27070.11747811335444495No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGCCATTGTGATGACTCAGTCTCCAGTCTCCCTGTCCGTCGCCC26760.11613277847672505No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26760.11613277847672505No Hit
GTCAGGGACCCCGGAGGCCCGATCAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCCTCTGCAGGTA26640.1156120036853496No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT26600.11543841208822446No Hit
TTATTACTGCATGCAAGCTCATCAAATTCCGTACACTTTTGGCCAGGGGA26370.11444026040475484No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC26000.11283453813134721No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25960.11266094653422207No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25180.10927591039028164No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC25070.10879853349818748No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24990.1084513503039372No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24810.10767018811687402No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24390.10584747634705995No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGCCATTGTGATGACTCAGTCTCCAGT24270.1053267015556845No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24260.10528330365640322No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTGATCGGGCCTCCGGGGTCCCT24230.10515310995855935No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24180.10493612046215291No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGCCATTGTGATGA24110.1046323351671839No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23980.10406816247652716No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGCCATTGTGATGACTCAGTCTCCAGTCTCC23760.10311340869233884No Hit
ATTTGGGTTCTGATCGGGCCTCCGGGGTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGATTTTACACTGA23690.10280962339736983No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23650.10263603180024466No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23570.10228884860599438No Hit
CCTCCATAGTAATGGATACAAGTATTTGGATTGGTACCTGCAGAGGCCAG23510.10202846121030666No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23440.10172467591533765No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23290.10107370742611835No Hit
CTGCAGGTACCAATCCAAATACTTGTATCCATTACTATGGAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGC23280.10103030952683704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)