FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304259
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69580.30196258319919766No Hit
GTAGTATATGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGGAGAAACA51440.22323879390294235No Hit
GGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTGATCGGGCC50190.2178140564927814No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT49430.2145158161474036No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46740.20284178124073726No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46320.20101906947092318No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44470.19299045810388502No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42630.18500524463612816No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG42400.18400709295265855No Hit
GATTTTACACTGACAGTCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCATCAAATT39320.170640539974022No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38540.1672555038300816No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37870.16434784457823534No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37800.16404405928326635No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA37560.16300250970051544No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGATTTTACACTGAC36280.15744757859251066No Hit
GTACCTGCAGAGGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTGATCGGGCCTCCGGGGTCCCTGA35800.15536447942700884No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT35590.15445312354210183No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA35140.1525002180744439No Hit
GGTCTAGTCAGAGCCTCCTCCATAGTAATGGATACAAGTATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTC34480.1496359567218789No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT33910.14716227646284552No Hit
GGCCAAAAGTGTACGGAATTTGATGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGA33800.14668489957075137No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33200.14408102561387415No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32840.14251870123974777No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32490.14099977476490275No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGACTGTCAGTGTAA32320.14026201047712084No Hit
GTGTAAAATCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG32160.13956764408862024No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31390.1362260058439611No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31380.13618260794467985No Hit
GTATAGTAGTAATATTGTGACTGGAAAAACGACTCCAAAAACTGCACTTT31230.1355316394554605No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC30330.13162582852014465No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC29770.12919554616039255No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29640.12863137346973583No Hit
ATATACTACTGTGCGAAAGTGCAGTTTTTGGAGTCGTTTTTCCAGTCACAATATTACTACTATACTATGGACGT29220.12680866169992178No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28530.12381420664951293No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28510.12372741085095035No Hit
GCCCTGGCCTCTGCAGGTACCAATCCAAATACTTGTATCCATTACTATGG28230.1225122696710743No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27350.11869325453432102No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27100.11760830705228882No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTC26640.1156120036853496No Hit
GTATATGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGGAGAAACAGCG26560.11526482049109932No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT26060.11309492552703494No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25570.11096842846225186No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25190.10931930828956293No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25190.10931930828956293No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25100.10892872719603135No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25090.10888532929675006No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24930.10819096290824946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24320.10554369105209094No Hit
GTCAGGGACCCCGGAGGCCCGATCAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCCTCTGCAGGTA24100.10458893726790261No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA23750.10307001079305755No Hit
CTGCAGGTACCAATCCAAATACTTGTATCCATTACTATGGAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGC23630.1025492360016821No Hit
TTATTACTGCATGCAAGCTCATCAAATTCCGTACACTTTTGGCCAGGGGA23590.10237564440455695No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGCCATTGTGATGACTCAGTCTCCAGTCTCCCTGTCCGTCGCCC23400.10155108431821248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)