FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030000
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCCTCTGGGATCCCA24620.23902912621359224No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20870.202621359223301No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC19270.18708737864077668No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18610.18067961165048543No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCACTACTGTCCCGGGAG18270.17737864077669904No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCA17630.17116504854368933No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17090.16592233009708737No Hit
GTAGTGGTGACCGCGTGCTTTTCGGCGGAGGGACCAACCTGACCGTCCTA16190.15718446601941746No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15420.14970873786407768No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15100.1466019417475728No Hit
CACCACTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGCCCCAGTGATGGTCAAGGACG14850.14417475728155338No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14720.1429126213592233No Hit
AGCCAGGACAGGCCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCC14680.14252427184466018No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14400.13980582524271845No Hit
GTATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAACTT14180.13766990291262135No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13570.13174757281553398No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTC12530.12165048543689322No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12370.12009708737864078No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11690.11349514563106797No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11410.11077669902912622No Hit
CTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGC11280.10951456310679612No Hit
GATCAGCACTGAGCACCGAGGATTCCCCATGGAACTGGGGCTCCGCTGGG11170.10844660194174757No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11100.10776699029126213No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10990.1066990291262136No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10980.10660194174757281No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10970.10650485436893205No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10720.10407766990291262No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10370.10067961165048543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2450.049.198321
GTATCAA21550.042.3493651
CACGAGA3150.041.52104621
GGTATCA8550.040.682121
TGGTATC3000.040.164972
TATCTCG1550.040.12991336
TATACAC3550.039.76093
CTTATAC3650.039.6282351
TATCAAC23750.038.703332
ACGAGAC3300.038.59260622
ACCGATC3050.038.40014627
TTATACA3900.037.9581072
CGATCAG3450.035.9588729
ATCAACG25750.035.6972433
TCAACGC25950.035.4221234
ATCAGTA3850.034.92000631
CAACGCA26350.034.8844035
TCCGAGC3850.034.86914413
AACGCAG26450.034.7525186
CGTTTGC501.8183234E-434.6373541
Produced by FastQC (version 0.11.5)