FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030000
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24210.23504854368932038No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23460.22776699029126213No Hit
CCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCCTCTGGGATCCCA22630.21970873786407766No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19300.18737864077669902No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC17700.17184466019417474No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCACTACTGTCCCGGGAG15930.1546601941747573No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15660.15203883495145631No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAAGCACGCGGTCACCA15290.14844660194174758No Hit
GTAGTGGTGACCGCGTGCTTTTCGGCGGAGGGACCAACCTGACCGTCCTA14560.14135922330097087No Hit
AGCCAGGACAGGCCCCTATACTTGTCATCTTTGGTAAAAACAATCGACCC13820.1341747572815534No Hit
CACCACTACTGTCCCGGGAGCAACAGAAATAGTCAGCCTCATCGTCCGCCTGAGCCCCAGTGATGGTCAAGGACG13370.12980582524271844No Hit
GTATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAACTT13340.1295145631067961No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13110.12728155339805824No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13060.12679611650485437No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13050.12669902912621359No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12620.12252427184466019No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTC11070.1074757281553398No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10870.10553398058252426No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10840.10524271844660193No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10730.1041747572815534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG200.007418561751.6442628
GGTATCA8950.042.2539061
TGGTATC3100.041.0074652
GTGGTAT3200.039.7510831
GTATCAA21800.039.7412261
ATATGCG350.001307518339.67236746
TATCAAC23650.036.6093752
ATCAACG25650.033.21263
TCAACGC25900.032.6267554
CAACGCA26350.032.3355
AACGCAG27000.031.5596316
ACGCAGA28650.029.8677927
CGCAGAG29150.029.3569078
AGAGTAC29900.029.19523611
ATGTGAT7700.029.18995770
CAGAGTA30200.028.6776210
AACACGC500.007505311627.77065846
TCCGAGA4500.025.73040670
GGATGGG13350.025.50932570
TACGGGC1951.8189894E-1224.67179315
Produced by FastQC (version 0.11.5)