FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580138
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24540.4230028027814072No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22480.38749401004588563No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18610.3207857440815806No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18450.31802777959726825No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17640.30406558439543696No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17080.2944127087003437No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16940.2919994897765704No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16390.28251898686174665No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACTCCCCGAAGTATTGGCAGTAATAAGTTG16000.2757964484312353No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15460.2664883182966811No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14400.2482168035881118No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14310.24666544856568612No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13610.23459935394681955No Hit
GTCTTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT12770.2201200404041797No Hit
GTAATACACGGCCGTGTCCGCAGTGGTCACAGAACTCAGCTTCAGGGAGA12510.21563834811717214No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12500.21546597533690262No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12340.21270801085259025No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGATAGAGTCACTATCACTTGCCG12210.21046716470908647No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA12200.21029479192881695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11860.2044341173996532No Hit
GTCTGCATCTGTAGGAGATAGAGTCACTATCACTTGCCGGGCCAGTCAGA11630.2004695434534542No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11390.19633259672698564No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11350.19564310560590756No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11200.1930575139018647No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11170.19254039556105615No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11070.19081666775836095No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10820.18650734825162288No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCGAAATGTGACAT10770.18564548435027528No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10680.1840941293278496No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10480.18064667372245913No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10440.17995718260138105No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAAGACTCTGA10300.17754396367760775No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10070.17357938973140874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10060.17340701695113922No Hit
GTACTAAGACTCTGACTGGCCCGGCAAGTGATAGTGACTCTATCTCCTACAGATGCAGACAGGGTGGAAGGAGA9740.1678910879825145No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9690.16702922408116688No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9580.16513312349820214No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9430.16254753179415932No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAATACTTCGGGGAGTTCGGCCAAGGGAC9400.16203041345335076No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA8880.1530670288793356No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8720.15030906439502326No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8660.14927482771340614No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8530.14703398156990233No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8450.14565499932774614No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8450.14565499932774614No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8380.1444483898658595No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8240.1420351709420862No Hit
GTCAGAGTCTTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGG8120.13996669757885194No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT8100.13962195201831287No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8070.1391048336775043No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC7920.1365192419734615No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7870.1356573780721139No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7810.13462314139049675No Hit
ACTTATTACTGCCAATACTTCGGGGAGTTCGGCCAAGGGACCAAGGTGGA7780.1341060230496882No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7780.1341060230496882No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7760.13376127748914915No Hit
GTGTATTACTGTGCGAGAGCCCTATTACGATACTTTGACTGGTTACCCGC7750.1335889047088796No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7740.13341653192861008No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG7710.13289941358780152No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7670.13220992246672345No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7630.13152043134564534No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG7610.1311756857851063No Hit
GTAGGAGATAGAGTCACTATCACTTGCCGGGCCAGTCAGAGTCTTAGTACCTGGTTGGCCTGGTATCAGCAGAAA7440.12824534852052444No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT7400.12755585739944636No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7200.1241084017940559No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGATAGAGTCACTATCACTTGCCGGGCCAGTCAGAGTCTTAGTAC7180.12376365623351686No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7170.12359128345324734No Hit
GTATTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGG7150.1232465378927083No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7120.12272941955189971No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7070.12186755565055211No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCGAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC7010.12083331896893497No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6970.12014382784785689No Hit
TTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC6890.11876484560570072No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6870.11842010004516168No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6870.11842010004516168No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6830.11773060892408357No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC6830.11773060892408357No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA6790.1170411178030055No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6680.11514501722004074No Hit
CCTTGGTCCCTTGGCCGAACTCCCCGAAGTATTGGCAGTAATAAGTTGCA6670.11497264443977122No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6650.11462789887923218No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6640.11445552609896266No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6530.11255942551599793No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC6500.11204230717518934No Hit
CCCCGAAGTATTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCC6430.1108356977133027No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6290.10842247878952939No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6270.10807773322899035No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG6230.10738824210791224No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6200.10687112376710368No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6130.10566451430521703No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6100.10514739596440847No Hit
GTCTCTGGTGCCTCCGTCAGTAGTTATTACTGGACCTGGGTCCGGCAGTC6090.10497502318413895No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC5980.1030789226011742No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC5970.10290654982090468No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC5960.10273417704063516No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT5890.10152756757874849No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5870.10118282201820945No Hit
GTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5850.10083807645767041No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA5840.1006657036774009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1600.053.728051
AGTTAAC200.00667088753.04738665
GAACGGT200.00708497752.24574351
CATACAC200.007466869451.5566983
TGTGTAT408.4793464E-751.55225412
GCTTGTC950.047.054461
CGTACTA751.8189894E-1145.8954329
ACCGTAC751.8189894E-1145.8756327
CTTATAC851.8189894E-1244.499471
CGTATGC803.274181E-1143.28479441
GTACTAG803.45608E-1143.03811330
ATGTGTA503.9418755E-641.24180211
TCGTATG856.366463E-1140.7138840
TACTAGA856.730261E-1140.52395631
GACCGTA856.91216E-1140.47500226
CACGAGA856.91216E-1140.443621
CTTGAAA901.05501385E-1038.87032357
ACTAGAT801.6625563E-938.7543832
ACGAGAC901.2732926E-1038.1967322
TTATACA1009.094947E-1237.8082432
Produced by FastQC (version 0.11.5)