FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204041
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCTCCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT78410.35575563249503983No Hit
CTTTTATACAGCTCCAACAATGAGAACTACTTAGCTTGGTACCAGCAGAA72660.3296671885867822No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTCATTGTTGGAGCTGTATAAAAGACT67140.3046222824348549No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61270.27798938404503365No Hit
CTCCTAAGGTGCTCATTTCCTGGGCATCGACCCGGGAGTCCGGGGTCCCT59940.2719550135410367No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC44860.20353523369120627No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44220.20063147645620022No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40890.18552286459280928No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40260.18266447856460022No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36520.16569564722253352No Hit
ACCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC35420.16070481447486684No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGACAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA35420.16070481447486684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34540.15671214827673352No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33190.15058703535914258No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGAAGAGACA32940.14945275518921836No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC31850.14450729364834863No Hit
TTATAGTACTCCTCCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT31150.14133130917256076No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC31140.1412859379657638No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30000.13611362039090924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29710.1347978553937971No Hit
GTGTATTACTGTGCGAGGACCAAATATGATTACATCAGGGGGAGGTATCG29180.13239318143355772No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTGTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA28350.12862737126940924No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGACAGGGC27860.12640418213635773No Hit
GTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT26400.11977998594400015No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGTGGAGGAGTACTATAATACTG25990.11791976646532437No Hit
CTCCCGGGTCGATGCCCAGGAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTT24580.11152242630695164No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC24410.11075111579140315No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTCCTGGGCATCGACCCGGGAG23390.10612325269811224No Hit
GTCAGGGACCCCGGACTCCCGGGTCGATGCCCAGGAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA23200.10526119976896982No Hit
CTCCCTGGCTGTGTCTCTGGGCGACAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAA22720.10308338184271527No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22690.10294726822232436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22650.10276578339513648No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22580.1024481849475577No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22330.10131390477763344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)