FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204041
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCTCCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT70590.32027534877980945No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC68350.3101121984572882No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58680.2662382414846185No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTCATTGTTGGAGCTGTATAAAAGACT56480.25625657598928514No Hit
CTTTTATACAGCTCCAACAATGAGAACTACTTAGCTTGGTACCAGCAGAA49580.224950443299376No Hit
CTCCTAAGGTGCTCATTTCCTGGGCATCGACCCGGGAGTCCGGGGTCCCT45270.20539545316988206No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38880.17640325202661838No Hit
GTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT37940.1721383585877032No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36560.1658771320497214No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34590.15693900431071836No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGTGGAGGAGTACTATAATACTG31210.14160353641334258No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGAAGAGACA30360.13774698383560013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30060.13638584763169107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30050.1363404764248941No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28960.13139501488402439No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28410.12889959851019103No Hit
ACCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC27150.12318282645377286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26830.12173094783626984No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26470.12009758439157892No Hit
TTATAGTACTCCTCCACTCACTTTCGGCGGAGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCAT26140.11860033456727892No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25030.11356413061281527No Hit
GTGTATTACTGTGCGAGGACCAAATATGATTACATCAGGGGGAGGTATCG24330.1103881461370274No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGACAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA24130.10948072200108801No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23990.10884552510593043No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA23470.106486222352488No Hit
CTCCCGGGTCGATGCCCAGGAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTT22590.10249355615435465No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22510.10213058649997889No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22400.10163150322521222No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGTGGAGGAGTACTATAATACTGCTG22310.10122316236403951No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22300.10117779115724254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11100.043.6440431
GGTATCA28900.042.68011
TGGTATC11600.041.147092
GTATCAA62000.035.4109881
TATCAAC72900.030.1934572
ATCAACG76000.028.8616923
TCAACGC76200.028.7461074
CAACGCA77950.028.0611615
AACGCAG78550.028.0242256
TCGGGAG16250.026.130170
CGCAGAG84350.026.0170028
ACGCAGA85500.025.7474027
AGAGTAC85550.025.4909111
CAGAGTA85850.025.28239810
GCAGAGT88250.024.7894129
CGCGTAT750.00204789422.89394612
GCGTTGT13200.022.61292362
TGTTAGA12450.022.3817770
GTAATAC14150.022.3388211
AGTACGG65750.021.6732913
Produced by FastQC (version 0.11.5)