FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182552
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155751.3170668182033434No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC18450.15601850912264323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14600.050.24161
GCTTACC352.781178E-547.631641
TAGCGAT350.001123432840.9159450
TCCGTAA400.001935809936.62706859
GTATCAA112900.036.1772651
GGTATCA54100.034.45151
TGGTATC23100.032.461012
TATCAAC125850.032.2806242
TCAACGC135100.029.765614
ATCAACG136450.029.7115153
CAACGCA136600.029.4375135
AACGCAG137750.029.2643096
ACGCAGA149600.026.9028457
CGCAGAG150450.026.640138
CTCCGTA550.00931976126.56499558
ACGACTG1357.929775E-726.18960269
AGAGTAC155650.025.87855511
CAGAGTA156600.025.63646710
GCAGAGT156550.025.602099
ATAAACG801.21311234E-424.9844063
Produced by FastQC (version 0.11.5)