FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606955
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93161.5348749083539968No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC15640.25767972913972204No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC9070.14943447207783114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCG250.001001379885.5789570
TGCATAA150.001900627772.8309453
CTATACA150.001983415672.0524550
TCCAATT150.00226976869.64403541
TTACGCA150.002789712766.113913
AGCATAC150.002790624566.108466
GGGCTTA200.00594142954.6232153
GAGACTC250.006230686353.9690569
TTAGGGA200.00627918153.86516649
TTTACTG200.006337206353.74006348
GTGGTAT9750.051.908211
CTGATTA200.00860371549.74121522
TATCCTA200.00871672349.577265
CTAGACT352.9285999E-547.212544
TACACTG307.793457E-444.0686725
AGGTTAG358.842903E-442.9513863
TAGGTTA359.0945564E-442.7072762
GTAGGTT359.3007926E-442.5131861
ACCTAGA406.4259664E-541.3280032
CTGTATC505.126678E-639.6945151
Produced by FastQC (version 0.11.5)