FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703152
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81170.4765869399795203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10600.040.894321
GTATCAA64750.039.5354461
GGTATCA27750.037.145751
TATCAAC73400.034.441582
TGGTATC12800.032.783562
TCAACGC78700.031.6709634
ATCAACG79450.031.6369233
CAACGCA80750.030.8310435
AACGCAG84100.029.7651426
CGCAGAG88050.028.431528
ACGCAGA89100.028.0581917
AGAGTAC90700.027.52562511
CAGAGTA92650.026.98310710
GCAGAGT94350.026.4969259
GAGTACG60550.024.33341212
AGTACGG60650.024.01141213
GAGTACT46650.021.7870312
ATTAGCG953.2961427E-421.55785420
AGTACTT46150.021.50512313
GTACGGG65950.021.40634214
Produced by FastQC (version 0.11.5)