FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876206
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27130.3096303837225493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG200.00752794151.4527855
GGTATCA10050.039.614811
GTATCAA24050.037.53241
TATCAAC26450.034.25072
ATCAACG27050.033.717743
TCAACGC27200.033.5375374
CAACGCA27850.032.7622765
GTGGTAT3600.031.461331
AACGCAG28900.031.4514646
ACGCAGA30900.029.4157687
CGCAGAG31250.029.0863118
CGAATTA1254.4055923E-927.44461815
AGAGTAC33000.027.4399211
CGTGCAT500.00795658627.43991910
CAGAGTA33500.027.23514210
TGGTATC4250.025.8376052
GCAGAGT35650.025.5926329
TAATTCG851.3338843E-424.63645751
AGTACGG21600.024.2957613
CGAAATT1601.9736035E-923.94203248
Produced by FastQC (version 0.11.5)