FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903817
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGTTCCAGGGAGAACTGGCTCTCGGACGCGTCTAGTGA12590.13929811012627558No Hit
CCACTACACCTCGCACAGTAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGTTCCAGGGAGAACTGGCT12010.13288088185993405No Hit
GTCTCTAGTGACTCCATCACTGACTCCATCAGTCGTTACTACTGGAGCTGGATCCGGCAGGCCCCAGGGAAGGAA10840.11993578346058992No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT9870.10920352239446703No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT9060.1002415311949211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT350.001362012139.34372312
GGTATCA9650.038.1969261
TAGTACG557.5027165E-637.555377
GTATCAA20750.036.6898651
TATCAAC22950.033.6044582
TCAACGC24700.031.2235744
ATCAACG24950.030.9107133
CAACGCA25950.029.8522265
AACGCAG26650.029.1973086
GTGGTAT4500.029.089951
ACGCAGA27850.027.9361637
TTCGGGG6550.027.13862870
CGCAGAG28950.026.9935998
AGAGTAC28700.026.86888511
ACTACGC1705.456968E-1226.33723427
CAGAGTA30050.025.8909210
GCAGAGT30100.025.8479129
TTATACA2950.025.6762872
CGAGACT2150.025.62483823
AGTACGG23800.025.45770313
Produced by FastQC (version 0.11.5)