FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383246
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155270.6515063908635533No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC27300.11454965202920722No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT27030.11341674338276451No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT26440.1109411281923897No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA24740.10380799967775042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16950.048.96361
TGGTATC19450.042.821582
GTATCAA129200.039.4105531
GGTATCA51800.039.332391
TATCAAC153650.032.9220282
ATCAACG154350.032.7176783
TCAACGC155150.032.420284
CAACGCA157450.031.9075365
AACGCAG161600.031.1932966
CGCAGAG171950.029.3954018
GCAGAGT177000.028.6719429
AGAGTAC177050.028.49105611
CAGAGTA179200.028.2440710
ACGCAGA181700.027.8180477
ACCGATT3750.025.7502270
GCTTGTA6950.025.4490621
GAGTACG134000.025.26427812
AGTACGG135000.025.00107813
GTAGTTT15350.023.48554270
ACGGGTC6200.021.94198616
Produced by FastQC (version 0.11.5)