FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926476
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144871.5636670566749704No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC10880.11743423466986733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9400.052.9003221
GATTCGT200.00809199450.5210926
GCCGTTA450.001499777138.57880469
GTATCAA67750.036.1032681
GGTATCA34300.035.2641031
TGGTATC14300.034.9934732
TATCAAC77350.031.3916972
ATCAACG84650.028.5135463
TCAACGC85050.028.3035554
CGTTAGT500.007126867328.06619644
GTCTCTC606.271426E-428.04297825
AACGCAG86100.027.8849166
CAACGCA86650.027.7839265
CGTAGTA500.00883719326.8558733
AATCCGG700.001047828525.68348355
ACGCAGA94050.025.527827
CGCAGAG96650.024.8758358
CAGAGTA98150.024.63251110
AGAGTAC98350.024.4445311
GCAGAGT98800.024.3005229
Produced by FastQC (version 0.11.5)