FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254712
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT42300.18760710902323668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13950.042.320631
GTGGTAT13650.041.0007131
TCGTATG11800.040.78993640
CGTATGC12900.039.5241441
GGTATCA45250.038.537411
AGGGGGG15300.038.4061470
CTCGTAT9650.038.32268539
GTATCAA103100.038.1637341
TCTCGTA9850.037.1360838
TTATACA16000.037.0893552
TGGTATC15850.036.5796662
TATACAC16350.036.503183
ATGCCGT13950.035.4740744
ACGAGAC16900.034.51489322
ATCTCCG17500.034.2962410
GCCGTCT13400.033.9076146
TCTCCGA17750.033.81319411
TATGCCG14750.033.50449443
CCCACGA17350.033.21837219
TATCAAC120400.033.0854032
Produced by FastQC (version 0.11.5)