FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111267
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC6260.5626106572478812No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT4850.435888448506745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.4053313201578186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.2947864146602317No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC3160.2840015458311988No Hit
CAATTAAACTATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCT3090.2777103723475963No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.1941276389225916No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT2070.18603898730081694No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1900.17076042312635373No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1750.15727933709006264No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1680.15098816360646014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.15008942453737406No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC1600.14379825105377156No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1600.14379825105377156No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1570.14110203384651332TruSeq Adapter, Index 3 (95% over 21bp)
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1560.14020329477742727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.13391212129382477No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT1420.12762094781022226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.12492473060296404No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1320.11863355711936154No Hit
TCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGTCTTT1320.11863355711936154No Hit
ATTCTTAGGTAGAATCCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATAAGGTTGCTAGTT1180.10605121015215653No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCGTATGCCGT1180.10605121015215653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.10245625387581224No Hit
TCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCC1130.10155751480672617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.10155751480672617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGCG250.001024865784.9759870
GTACCCC150.002986932964.9477131
AGTACCC150.003064413864.5296530
ATAAGTA150.003177686863.94168527
AACCTCC200.003226804363.68058466
TTGCTTC150.0033534163.07954811
TAATAGT350.003893133560.69713270
TAGTGAT350.003893133560.69713270
CAGAATT200.00414713159.77512759
CGTGAAA351.04120645E-556.16326557
GGGTCCG200.005430346455.8429747
CCTACGG200.00674362152.86991542
CCACGTT200.00725334251.90532740
AGGCCTA200.00755509351.37335639
CTAGGCC200.00800224550.63203437
TATACAC1950.050.1401563
CCCCTAG200.00859655349.72319434
ACCCCTA200.00882357249.3965333
AGGGGGG3000.047.20887870
GCGTGAA501.5718833E-647.0716656
Produced by FastQC (version 0.11.5)