FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111267
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21071.8936432185643541No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5370.4826228800992208No Hit
CAATTAAACTATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCT2940.26422928631130527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.23007720168603452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.22738098447877628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.18873520450807518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.16446924964275123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1790.16087429336640693No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1730.15548185895189048No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG1690.15188690267554622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.15098816360646014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.1420007729155994No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.13301338222473869No Hit
GTATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA1470.13211464315565263No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC1440.1294184259483944No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1320.11863355711936154No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1310.11773481805027546No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1290.11593733991210331No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1230.11054490549758687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10964616642850081No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC1210.10874742735941476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10784868829032868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1150.1033549929448983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCC150.00106965284.1171665
TGTTAGT150.0026132967.1732737
TAAGGAG200.004716702757.86513554
GTAAGGA200.00476813657.70694753
TACCACT503.6379788E-1156.9492848
TCGTAGA252.3432737E-456.1788442
CTACCGC250.00716486952.05721367
CTGCTTG502.3701432E-950.62158613
ACTGCTT502.3701432E-950.62158612
TGCTTGT555.511538E-946.0196214
TTGTATC601.17506715E-842.2416317
ATACCAC701.0186341E-940.678067
CGTAGAA350.001157082240.63466343
GTGGTAT3500.039.7919921
GCTTGTA652.4097972E-838.93968215
GCTGCCA1300.036.4895443
CACTGCT704.629328E-836.1582711
ACCACTG704.629328E-836.158279
CTGCCAA1300.034.0569084
CTTGTAT758.425559E-833.778116
Produced by FastQC (version 0.11.5)