FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106174
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGAGATTGGCACTGGACCTGGATCCGCCAGCC45700.41313572729064324No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGACTTCAGGCTCAGGGAAA42300.3823991523937464No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33770.3052865100788845No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA30310.2740075250367483No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26900.2431805484489782No Hit
GTGTATTACTGTGCGAGGCCACAGGCCGGAGCCATTTTTGAGGTCTGGGGCCAAGGGACAACGGTCACCATCTC24820.22437699674734715No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22810.2062062568818287No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAATACCCCAATACTCAGGCTGTTATCCCATGCT20410.18450985107225445No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG17740.16037259960910308No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17120.15476769477496308No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16850.15232684912138597No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCACAGACTTCAGGCTCAGGGA16830.15214604573963952No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16610.15015720854042855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16090.14545632061502078No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT15970.14437150032454207No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15340.13867619379952884No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15000.13560253630983915No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14980.1354217329280927No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC14480.13090164838443138No Hit
CCCTGAGGAGATGGTGACCGTTGTCCCTTGGCCCCAGACCTCAAAAATGG13970.12629116214989683No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13200.11933023195265845No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13070.1181550099713065No Hit
GTATTGGGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCC13010.11761259982606714No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12950.11707018968082779No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12860.11625657446296875No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12510.11309251528240584No Hit
CCCCAATACTCAGGCTGTTATCCCATGCTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC12230.11056126793795552No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12130.10965725102922326No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12090.10929564426573035No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11410.10314832928635097No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC11300.1021539106867455No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11270.1018827056141258No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA11220.10143069715975968No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11170.10097868870539355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3000.050.5226171
TGTTGAT14750.043.67506470
TGGATTG17250.042.06265670
GTATCAA22350.039.302221
GGATGGG13600.035.90009370
ATCAACG26850.033.199893
TCAACGC26850.033.076194
CAACGCA27650.032.2422335
AACGCAG28250.031.3151686
CTCGCTA450.00465632230.62115123
GTGGATT17100.029.84276669
ACGCAGA29850.029.6366337
CGCAGAG29700.029.5545128
AGAGTAC30250.028.78957411
TCCGAGA6550.027.95274570
CAGAGTA31200.027.91296810
AAGGGGC16400.027.87144358
GCAGAGT31400.027.7351789
AATGGTA2953.45608E-1127.58425370
GCTCGGG3004.3655746E-1127.12451470
Produced by FastQC (version 0.11.5)