FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629091
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT27020.10277316380452409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6700.042.9140941
TCGTATG6850.040.92840640
GTATCAA79100.039.775391
GTGGTAT12100.039.50021
GGTATCA34600.037.6645661
CTCGTAT6450.037.5356939
TGGTATC14050.034.9809232
TATCAAC92250.034.0526852
ATCAACG94750.033.1179243
TCAACGC95250.033.0162434
TATACAC10200.032.6845973
CAACGCA97200.032.353885
ACGGACT9300.032.17830727
CGAGACG9400.031.4526923
TATCTCG7150.031.38756236
ACGAGAC9550.031.3150822
AACGCAG101100.031.17386
GACGGAC9750.030.6884826
ATGCCGT9800.030.46860144
CTTATAC10550.029.9850791
Produced by FastQC (version 0.11.5)