FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629091
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64530.24544604960421681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12000.040.619261
GTATCAA80000.039.775411
GGTATCA34700.037.6895031
TATCAAC93150.034.138212
TCAACGC95200.033.2121774
ATCAACG95600.033.0732153
CAACGCA96500.032.802795
TGGTATC15100.032.713682
AACGCAG100050.031.6749616
ACGCAGA106050.030.0128177
CGCAGAG106750.029.7196258
AGAGTAC110300.028.79310211
CAGAGTA111850.028.42583510
GCAGAGT117600.026.9193069
AGTACGG87150.024.90804713
GAGTACG88050.024.81207312
GTACGGG96550.022.12700514
ACGCTAA2353.5632474E-622.03744370
GAGTACT46150.021.625912
TACGGGG44200.021.49898115
Produced by FastQC (version 0.11.5)