FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737867
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46820.6345316974468299No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39200.5312610538213527No Hit
GACCAGGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAA35710.4839625569377679No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34280.4645823705356114No Hit
CTTTTATTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAG34080.4618718549548903No Hit
TCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT30790.41728387365202674No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30500.413353626059981No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30380.41172731671154833No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30290.4105075847002237No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29330.3974971099127621No Hit
ATATTACAGTTCTCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCAT27730.3758129852669926No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27640.374593253255668No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG27040.36646170651350446No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26010.3525025512727904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23630.32024741586220823No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23280.31550401359594615No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAATCCGGGGTCCCTGA23280.31550401359594615No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22630.3066948379586023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22120.2997830232277633No Hit
CTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAATCCGGGGTCCCT21970.2977501365422224No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCTCCACCC21870.29639487875186177No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21320.28894096090487853No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21300.2886699093468064No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAAAAGAGT20660.2799962594884986No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGCCAGACTCTTTTATT20610.2793186305933183No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20250.2744397025480202No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20200.2737620736528399No Hit
CTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTC20070.2720002385253711No Hit
GTTTATTACTGTCAGCAATATTACAGTTCTCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGCTCAAACGAA19130.2592608152959815No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18850.2554660934829719No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18690.2532976810183949No Hit
GTCAGGGACCCCGGATTCACGGATAGATGCCCAGGAAATGAGGAGCTTAGGAGGCTGTCCTGGTCTCTGCTGGTA18530.25112926855381795No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17880.2423200929164741No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17750.24055825778900533No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17600.23852537110346442No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17550.23784774220828417No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16920.2293096181290124No Hit
GAATAAAAGAGTCTGGCTGGACTTGCAGTTGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16820.2279543603386518No Hit
GGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16810.22781883455961577No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAAAAGAG16790.22754778300154363No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16280.2206359682707046No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGCCAGACTCTTTTATTCAGCTCCAA15870.21507941133022618No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGC15850.21480835977215404No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15840.214672833993118No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15740.2133175762027574No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15580.21114916373818046No Hit
GTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGCCAGACTCTTTTATTCAGCTCCAACAATAAGAACTA15520.2103360090639641No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14930.20233998810083662No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14320.19407291557963696No Hit
GTCTGGGACAGATTTCACTCTCACCATCTCCACCCTGCAGGCTGAAGATG14110.19122687421987974No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14010.18987161642951914No Hit
CACCATCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTTCTCCTCTCACTTT13940.18892293597626672No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13750.18634794617458159No Hit
CCTCATTTCCTGGGCATCTATCCGTGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13740.18621242039554553No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13720.18594136883747342No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13700.1856703172794013No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13660.18512821416325706No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCGCCGCCGAAAGTGAGAGGAGAA13620.1845861110471128No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13540.18350190481482434No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13440.18214664702446376No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13260.1797071830018147No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13230.1793006056647065No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13050.17686114164205743No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13020.17645456430494927No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12820.17374404872422808No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTG12660.17157563625965114No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTTCTCCTC12540.16994932691121842No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12470.169000646457966No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCGCCGCCGAAAGTG12370.1676453886676054No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12280.1664256566562809No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12250.1660190793191727No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12080.1637151410755597No Hit
GATTTCACTCTCACCATCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTTCT12050.1633085637384515No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11930.1616822543900188No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11680.15829410991411733No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11590.15707437790279277No Hit
CCATCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAA11450.15517701699628794No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11210.15192439829942253No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11080.15016256317195376No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCTC10990.1489428311606292No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10880.14745204759123257No Hit
GCAGAGACCAGGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAATCCGGGGTCCCTGACCGATT10670.14460600623147532No Hit
GGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAT10650.1443349546734032No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10620.14392837733629502No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10530.1427086453249705No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10460.14175996487171807No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10320.13986260396521324No Hit
TCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10270.13918497507003294No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCACGGATAGATGCCCAGGAAATGAGGAGCTTAGGAGGCTGTCCTGGT10220.13850734617485264No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10180.13796524305870841No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAGCTCCTCATT10070.13647445948931175No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9940.13471262436184298No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9930.13457709858280695No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9790.1326797376763021No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9720.13173105722304967No Hit
TTATTACTGTCAGCAATATTACAGTTCTCCTCTCACTTTCGGCGGCGGGA9630.13051132521172515No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCACGGAT9560.12956264475847273No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9460.12820738696811215No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9450.1280718611890761No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9360.12685212917775154No Hit
TTACAGTTCTCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTT9300.1260389745035352No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9270.125632397166427No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC9220.12495476827124673No Hit
GTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGGTGG9160.12414161359703035No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9050.1226508300276337No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9050.1226508300276337No Hit
ATCCGTGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCTCCACC9030.12237977846956158No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9010.12210872691148947No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAATC8990.12183767535341736No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8980.1217021495743813No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGC8970.12156662379534523No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8930.121024520679201No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8920.12088899490016493No Hit
GGTCTGGGACAGATTTCACTCTCACCATCTCCACCCTGCAGGCTGAAGAT8920.12088899490016493No Hit
GGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAA8900.12061794334209282No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG8890.12048241756305675No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8840.11980478866787646No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8760.11872058243558797No Hit
GGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAATCCGG8690.11777190198233557No Hit
GGAAATGAGGAGCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTA8590.11641664419197498No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8550.11587454107583074No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC8520.11546796373872258No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTAT8390.11370612861125379No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG8370.11343507705318166No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA8220.11140219036764078No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG8210.11126666458860471No Hit
AGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8200.11113113880956867No Hit
CAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT8190.1109956130305326No Hit
TATTCATTCTCCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGG7840.10625221076427052No Hit
GAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAGCTCC7700.10435484985776569No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG7670.10394827252065751No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7650.1036772209625854No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA7600.1029995920674051No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC7510.10177986005608056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)