FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737867
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45420.6155580883817816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30890.41863913144238735No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30160.408745749572755No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29600.4011563059467356No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28650.38828135693831No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27420.37161168611687473No Hit
ATATTACAGTTCTCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCAT25260.34233811784508594No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25010.3389499733691844No Hit
GACCAGGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAA24760.33556182889328295No Hit
TCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT24120.32688817903497513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23790.32241582832678517No Hit
CTTTTATTCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAG23130.31347112691040524No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCGCCGCCGAAAGTG22970.3113027144458283No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG22890.3102185082135398No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22880.3100829824345038No Hit
CTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTC22640.30683036373763833No Hit
GGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAT22230.3012738067971599No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21650.2934133116130685No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA21540.2919225280436718No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21530.2917870022646358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21360.28948306402102275No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19960.27050945495597445No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19840.26888314560754173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19510.26441079489935176No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19020.25777003172658486No Hit
GTTTATTACTGTCAGCAATATTACAGTTCTCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGCTCAAACGAA18970.25709240283140455No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18960.2569568770523685No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCACGGAT18460.25018058810056554No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCGCCGCCGAAAGTGAGAGGAGAA18290.24787664985695254No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17800.24123588668418564No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17460.23662801019695962No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17180.23283328838394995No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16950.22971619546612057No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16910.22917409234997638No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16880.22876751501286818No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16660.22578594787407485No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16550.2242951643046782No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAAAAGAGT16190.2194162362593801No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAATCCGGGGTCCCTGA16010.216976772236731No Hit
CTCCTAAGCTCCTCATTTCCTGGGCATCTATCCGTGAATCCGGGGTCCCT15950.21616361756251465No Hit
GGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15820.21440178243504587No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCTCCACCC15650.21209784419143288No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15640.2119623184123968No Hit
GTCAGGGACCCCGGATTCACGGATAGATGCCCAGGAAATGAGGAGCTTAGGAGGCTGTCCTGGTCTCTGCTGGTA15240.20654128725095441No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGAATAAAAGAG15090.20450840056541356No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14520.19678343116035818No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14260.19325976090542063No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14230.19285318356831246No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGCCAGACTCTTTTATT14230.19285318356831246No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14190.19231108045216821No Hit
TCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC13790.18689004929072583No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13660.18512821416325706No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13350.18092691501313923No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13240.17943613144374257No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13010.1763190385259132No Hit
CCTTGGTCCCGCCGCCGAAAGTGAGAGGAGAACTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC12940.1753703580726608No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12830.17387957450326413No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12680.17184668781772325No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12280.1664256566562809No Hit
GTCTGGGACAGATTTCACTCTCACCATCTCCACCCTGCAGGCTGAAGATG12220.1656125019820645No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11950.16195330594809093No Hit
CACCATCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTTCTCCTCTCACTTT11660.1580230583560452No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11590.15707437790279277No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGCCAGACTCTTTTATTCAGCTCCAA11540.15639674900761247No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11530.15626122322857644No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11400.15449938810110767No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11390.15436386232207158No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11370.15409281076399947No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTTCTCCTC11360.15395728498496342No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11120.15070466628809798No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11030.14948493427677345No Hit
GATTTCACTCTCACCATCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACAGTTCT10940.14826520226544893No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10850.1470454702541244No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG10840.14690994447508832No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10610.14379285155725896No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10480.14203101642979019No Hit
GTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGCCAGACTCTTTTATTCAGCTCCAACAATAAGAACTA10360.1404047070813575No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10270.13918497507003294No Hit
GAATAAAAGAGTCTGGCTGGACTTGCAGTTGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA10220.13850734617485264No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10060.1363389337102757No Hit
ACCTTGGTCCCGCCGCCGAAAGTGAGAGGAGAACTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC9950.13484815014087906No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9920.13444157280377086No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9850.13349289235051848No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAAGTCCAGC9810.13295078923437423No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9800.13281526345533817No Hit
CCTCATTTCCTGGGCATCTATCCGTGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT9640.1306468509907612No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9640.1306468509907612No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9630.13051132521172515No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9490.12861396430522032No Hit
GTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGGTGG9480.12847843852618426No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9330.12644555184064338No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9200.12468371671317459No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8840.11980478866787646No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8820.11953373710980435No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8790.11912715977269617No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8790.11912715977269617No Hit
CCATCTCCACCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAA8780.11899163399366011No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8740.11844953087751586No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTG8660.11736532464522739No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA8650.11722979886619132No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCACGGATAGATGCCCAGGAAATGAGGAGCTTAGGAGGCTGTCCTGGT8610.11668769575004709No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8560.11601006685486678No Hit
TTATTACTGTCAGCAATATTACAGTTCTCCTCTCACTTTCGGCGGCGGGA8480.11492586062257834No Hit
GTGGAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCG8410.1139771801693259No Hit
GCCACAGTTCGTTTGAGCTCCACCTTGGTCCCGCCGCCGAAAGTGAGAGG8340.1130284997160735No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCTC8310.11262192237896532No Hit
GAACTGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGGTGGAGATGGTGAGAGTGAAAT8180.11086008725149654No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8170.11072456147246047No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8150.11045350991438836No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8120.1100469325772802No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC8100.10977588101920806No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8080.10950482946113596No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7960.10787852011270324No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCGCCGC7830.10611668498523447No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7790.10557458186909023No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7780.10543905609005416No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7780.10543905609005416No Hit
TTACAGTTCTCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTT7710.10449037563680175No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7660.10381274674162144No Hit
AGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7640.10354169518354935No Hit
GAGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC7510.10177986005608056No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC7510.10177986005608056No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7430.10069565382379209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.054.4892271
CGATTGC200.006703980752.9825163
CATAACT200.007160404752.10742647
TGGTATC2300.052.0918582
GTATCAA17950.045.9851841
TATCAAC20250.040.232922
GGTATCA6100.039.865191
CTAGGTC350.001403939439.1008343
TCAACGC22450.036.5803534
ATCAACG22450.036.2705963
CAACGCA22900.035.8639535
AACGCAG23250.035.326466
CGCAGAG24950.032.9194458
AGAGTAC25300.032.86761511
ACGCAGA24950.032.7822847
CAGAGTA25400.032.60569410
GCAGAGT25700.032.0919279
CCAACAC751.7804796E-631.9323483
GCTTGAT652.4384797E-531.6157281
GTGTCCA1801.7441562E-731.12911670
Produced by FastQC (version 0.11.5)