FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760499
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT22750.2991456924992669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21800.2866538943509459No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC17210.22629878540274215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.18882339095777903No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC9220.12123618834475784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.11387260206785282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATG150.00232419469.23093440
GTGGTAT8650.052.9242971
AAGTACG1900.048.5356521
TAGTACG352.989664E-547.0498661
CTTATAC8500.046.1088681
TTATACA8700.045.034082
AGGGGGG10800.043.5053170
TATACAC9350.042.9597553
ATACGGG706.9849193E-1042.344881
ACGTTAA350.001032596241.6210151
CACGTTA350.001052709541.45815350
TGAGACT400.001533384438.40468665
GGCTATA350.001692824837.6398931
TACGGAT350.001695561837.6275184
GTATCAA86150.037.5415841
TAGGGGG750.001745224937.40157770
ATATAAG451.3158779E-436.594341
ACGTGAC451.3184268E-436.582313
CGTGAAA2750.036.3807957
CAATCTA400.002029056736.27588750
Produced by FastQC (version 0.11.5)