FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940227
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTGGATAAAACACT50780.5400823418174547No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43160.45903808335646606No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGCAATTCGGGGTCCCT41930.44595613612457413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37190.39554277849923475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34880.37097424345397445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29360.31226501685231334No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28750.30577722188365153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28080.2986512831475803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27120.2884409828690306No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26830.285356621326552No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA25260.26865852607934043No Hit
ATATTATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25060.26653138018797584No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24620.26185165922697395No Hit
CTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC24300.2584482258007907No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23530.2502587141190372No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23090.24557899315803522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22890.2434518472666707No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22460.23887848360023695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20760.22079774352363846No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20430.21728795280288699No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20110.2138845193767037No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20030.2130336610201579No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19910.21175737348533918No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19510.20750308170261011No Hit
GTGTACTACTGTGCGAGAGAGATCATATGTTATGAGTATGTTTGCTGGTT19420.20654586605149608No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19070.20282336074160814No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTCACTGCAAGTCCAGCCAGAGTGTTTTATC19000.20207885967963055No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18520.19697370954035567No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18370.19537835012183227No Hit
GTTTTATCCAGTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA18240.19399570529244534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17990.19133677292823967No Hit
GTCAGGGACCCCGAATTGCCGGGTAGATGCCCAGTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTCTGCTGGTA17990.19133677292823967No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17570.18686976655637416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16910.1798501851148712No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGCAA16830.1789993267583254No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTG16610.1766594662778244No Hit
GTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16180.17208610261139065No Hit
ATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16130.17155431613854952No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTCACTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAA15740.16740638165038868No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15490.164747449286183No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC15330.16304573257309138No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15330.16304573257309138No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15210.16176944503827267No Hit
TTATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT15190.16155673044913624No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15190.16155673044913624No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAATATTATAGTAGT14770.1570897240772707No Hit
GTCTCTGGGCGAGAGGGCCACCATTCACTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAACAATAAGAACTA14610.15538800736417907No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14420.15336721876738277No Hit
GGATAAAACACTCTGGCTGGACTTGCAGTGAATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA14240.15145278746515467No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14230.15134643017058647No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14000.14890021239551726No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGC13880.14762392486069853No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAACAATAAGAACTACT13730.14602856544217513No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13710.1458158508530387No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13580.14443320602365173No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTCACTGCAAGTCCAGC13530.1439014195508106No Hit
GGCCAAAAGTGCGAGGACTACTATAATATTGCTGACAGAAATAAACTGCC13480.1433696330779695No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13450.1430505611942648No Hit
GCTCATTTACTGGGCATCTACCCGGCAATTCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13260.1410297725974685No Hit
CCACTGAATCGGTCAGGGACCCCGAATTGCCGGGTAGATGCCCAGTAAAT13110.1394344131789451No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13020.13847719752783105No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12900.13720090999301232No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12880.13698819540387586No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12810.13624369434189829No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12570.13369111927226085No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12560.13358476197769262No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12450.13241483173744212No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGAATTGCCGGGT12440.1323084744428739No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12250.13028768584607758No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12250.13028768584607758No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12240.13018132855150938No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12210.12986225666780468No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12140.12911775560582708No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12020.12784146807100838No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11920.12677789512532614No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11890.12645882324162144No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11740.12486346382309804No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11690.12433167735025691No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11680.12422532005568869No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11650.123906248171984No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11650.123906248171984No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11640.12379989087741576No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11610.12348081899371109No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAATATTATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAG11250.11965195638925492No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10960.11656759484677637No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10930.11624852296307167No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAACCTGCTCATT10820.11507859272282119No Hit
CTACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT10790.11475952083911652No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTCACTG10630.11305780412602488No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10580.11252601765318375No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG10500.11167515929663793No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10500.11167515929663793No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10400.11061158635095568No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10380.11039887176181923No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG10090.10731451021934064No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9740.10359200490945271No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9730.10348564761488449No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9720.10337929032031626No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9660.1027411465529069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT100.009108991133.3389970
CGCGTAT200.007309900651.83698739
ATGATCG200.00750378851.4949223
TCGTATG506.800474E-848.42788340
ATCTCGT451.8546089E-646.02308337
CGTATGC551.43129E-744.05606541
GGTATCA6400.043.4280361
CTCGTAT404.951978E-543.1974939
TGGTATC1850.042.6532362
GTATCAA16400.041.8457451
CTACGCT1450.040.2896428
ACTAATC350.001381550239.23004522
GTGGTAT1950.038.7126771
CTTATAC1650.037.432921
ACGAGAC1500.036.6147122
TACGCTG1600.036.52026729
ACTACGC1600.036.49887527
TATCAAC19150.035.830852
CGAGACT1550.035.43736323
GTGTATA807.4451236E-834.30986413
Produced by FastQC (version 0.11.5)