FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940227
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTGGATAAAACACT44380.4720136732937897No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36110.38405619068586627No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGCAATTCGGGGTCCCT35590.3785256113683185No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30790.32747410997556975No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29040.30886158342613007No Hit
ATATTATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28110.298970355031285No Hit
CTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27090.28812191098532586No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26910.2862074796830978No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25620.27248738868379657No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25550.27174288762181903No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24290.2583418685062224No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24040.25568293614201676No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22530.23962298466221454No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGAATTGCCGGGT21650.23026354274021063No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21250.22600925095748156No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA20770.22090410081820666No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20640.2195214559888197No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20420.21718159550831875No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20180.2146290204386813No Hit
GTGTACTACTGTGCGAGAGAGATCATATGTTATGAGTATGTTTGCTGGTT19780.21037472865595222No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19500.2073967244080419No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19300.20526957851667735No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18400.19569742200553694No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18100.19250670316849017No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18000.1914431302228079No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17690.18814605409119287No Hit
GGCCAAAAGTGCGAGGACTACTATAATATTGCTGACAGAAATAAACTGCC17670.18793333950205643No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17660.18782698220748817No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17410.18516804984328253No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17330.18431719148673673No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC17090.1817646164170993No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16880.17953111323116652No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16350.17389417661905052No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15860.1686826691852074No Hit
GTCAGGGACCCCGAATTGCCGGGTAGATGCCCAGTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTCTGCTGGTA15840.16846995459607095No Hit
GTTTTATCCAGTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA15670.16666188058841108No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTG15580.16570466493729707No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15500.16485380658075124No Hit
ATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA15020.15974865644147634No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14940.15889779808493054No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG14840.1578342251392483No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14790.15730243866640714No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAATATTATAGTAGT14570.15496257818590617No Hit
TTATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT14560.15485622089133794No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGC14530.15453714900763327No Hit
GTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA14450.15368629065108746No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGCAA14300.15209093123256406No Hit
CTACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14160.15060192910860887No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTCACTGCAAGTCCAGCCAGAGTGTTTTATC14150.15049557181404066No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13930.14815571133353966No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13910.14794299674440323No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13460.143156918488833No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13450.1430505611942648No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13450.1430505611942648No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13410.1426251320159919No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13410.1426251320159919No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13060.13890262670610395No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12920.13741362458214876No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAATATTATAGTAGTCCTCGCACTTTTGGCCAGGGGACCAAG12880.13698819540387586No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12850.1366691235201712No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA12840.13656276622560296No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11950.12709696700903078No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11890.12645882324162144No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11810.12560796488507564No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT11760.1250761784122345No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11720.12465074923396159No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11660.12401260546655224No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11190.11901381262184557No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11160.1186947407381409No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11140.11848202614900445No Hit
GACTACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT11100.11805659697073152No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11050.1175248104978904No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10960.11656759484677637No Hit
GCTCATTTACTGGGCATCTACCCGGCAATTCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT10960.11656759484677637No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10910.11603580837393522No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10910.11603580837393522No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10880.11571673649023057No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATTCACTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAA10790.11475952083911652No Hit
GTCTCTGGGCGAGAGGGCCACCATTCACTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAACAATAAGAACTA10590.11263237494775198No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10370.110292514467251No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10350.11007979987811455No Hit
ACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT10270.10922894152156873No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10230.10880351234329583No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10200.10848444045959114No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10120.10763358210304534No Hit
CCACTGAATCGGTCAGGGACCCCGAATTGCCGGGTAGATGCCCAGTAAAT10090.10731451021934064No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9730.10348564761488449No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9680.10295386114204336No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9620.102315717374634No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAACAATAAGAACTACT9580.1018902881963611No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9580.1018902881963611No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9560.10167757360722465No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG9550.1015712163126564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)