FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112655
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT8920.791797967245129No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5010.4447206071634637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.4056633083307443No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT3220.28582841418490085TruSeq Adapter, Index 12 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.26985042829878836No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC3040.26985042829878836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.1908481647507878No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.18907283298566419Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.17398251298211354No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT1910.1695441835693045RNA PCR Primer, Index 12 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.1659935200390573No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT1630.14468953885757402No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1580.140251209444765Clontech Universal Primer Mix Long (95% over 23bp)
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1560.1384758776796414No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1530.13581288003195596Clontech Universal Primer Mix Long (95% over 22bp)
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1530.13581288003195596No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1500.13314988238427056Clontech Universal Primer Mix Long (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.13137455061914693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.12516088944121434No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1360.12072256002840531No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.1171718964981581No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT1280.11362123296791088No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC1240.11007056943766368No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.10740757178997824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10651990590741646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.10563224002485465No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.10474457414229284No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT1180.10474457414229284No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10296924237716923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1140.10119391061204563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.10119391061204563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)