FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112655
Sequences flagged as poor quality0
Sequence length20-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25422.256446673472105No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5670.5033065554125428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.25742310594292306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.23789445652656338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.22635480005325995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.18374683769029337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.18108384004260797No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1840.16333052239137189No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG1760.15622919533087745No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1660.14735253650525942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.1384758776796414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1520.13492521414939418No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1500.13314988238427056No Hit
GTATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA1490.13226221650170875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1450.12871155297146153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.12871155297146153No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1420.12604855532377612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.12249789179352892No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1370.12161022591096711No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC1330.1180595623807199No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.11539656473303447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.10918290355510187No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10829523767254005No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.10740757178997824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1210.10740757178997824No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10651990590741646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAGA150.00116862282.265964
TGGACCC150.00220116870.1429340
ATAGGGC150.003334721363.1691063
CAGGACA200.004290017859.2670760
AGTCTCA200.00439479558.9072859
GCAGTCT200.004570343558.32786657
AGAGTGC250.005754841555.0206268
ATTAGAG250.00844658949.93684465
ACGGGTA601.1981683E-842.15015416
TGCTTGT752.0354491E-937.90146614
TCTCGGA400.001855961236.9087147
ACCACTG951.4551915E-1136.571599
CTTGTAT803.792593E-935.56419416
CGATGGC400.002294157435.35022441
CCACTGC1002.5465852E-1134.74300810
AGGGGAT552.0224911E-433.97631549
CGCTCAG400.00295132333.5818835
GTGGTAT4300.032.3477941
CTAGCAT400.003960379431.62666517
TACCACT1300.031.5845558
Produced by FastQC (version 0.11.5)