FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149624
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22181.4823825054804043No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC4790.3201358070897717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.1998342511896487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.19248248944019675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.16441212639683472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.15505533871571406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.15438699673849116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2000.1336683954445811No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1980.13233171149013528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.12096989787734587No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.11361813612789393No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1590.10626637437844197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1580.10559803240121905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATGG200.004675063358.00734760
GTTAATG200.004742545557.7978559
ACTGTTA200.00506710356.83987456
AGTAGAC200.005466338255.76161251
CAGTAGA200.005550608555.54656650
CTCCTGG252.4997402E-455.4636840
TTTAATG200.006400249453.58342444
AGGAGTA301.3864816E-553.54162
CCACGGT200.00827098550.22044834
AGCCTGG450.00947324648.4957170
ATAAGAG200.00973884148.1874353
AATTATG200.00973884148.1874352
TATAAGA200.00973884148.1874352
TATGCAG200.00975172548.171325
GTATAAG353.4364053E-545.9235271
GGGTGCT308.8401366E-442.93382618
TAGGGCC407.615844E-540.1293454
GACTTAT400.001252468739.99229464
TAGATCT751.7425918E-938.5241744
GCGGTCC350.001911227736.701969
Produced by FastQC (version 0.11.5)