FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744160
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA45140.2588065315108706No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45020.2581185212365838No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT37620.21569122098890012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34130.1956815888450601No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT31870.1827240620126594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29530.16930786166406753No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29050.16655582056692048No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28410.16288643243739107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28160.16145307769929365No Hit
ATATTATGGTATTCCGGGGTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT27510.15772635538024035No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27410.15715301348500138No Hit
CCATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGCCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTC27220.15606366388404733No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAACCCC23680.13576736079258783No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23260.13335932483258417No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGTTTCAGGGAGA22820.1308366204935327No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22750.13043528116686542No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22650.12986193927162645No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22270.12768324006971837No Hit
GTGATAAACTGCCACATCTTCAGCCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA21890.1255045408678103No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21870.1253898724887625No Hit
GTTTATCACTGTCAGCAATATTATGGTATTCCGGGGTTCGGCCCTGGGAC20850.11954178515732501No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19660.11271901660398129No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19650.1126616824144574No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19600.11237501146683791No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT19010.10899229428492799No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18810.10784561049445006No Hit
GTATATTACTGTGCGAAACAGGAACTGGGGATTAATTGGTTCTTCGATCTCTGGGGCCGTGGCACCCTGGTCA17930.10280020181634712No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA17840.10228419411063205No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17710.10153884964682139No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17530.10050683423539124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)