FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534072
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT22910.14934110002659587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9500.042.4181751
TCGTATG6300.040.08600640
GACGCGT6000.039.85102526
ACGCGTA6050.039.53973827
GTATCAA66250.038.248251
GGTATCA30150.035.566071
AGACGCG6800.035.15005525
GCGTAGT7250.034.92170329
CGCGTAG7050.034.91896428
TCTCGTA6050.034.77956438
CGTAGTA7400.034.69544630
CGAGACG6900.034.6327323
CTCGTAT6150.034.26822739
CGTATGC7400.034.1769641
AATCTCG6100.033.84648536
TATCAAC76850.033.446172
ACGAGAC7200.033.18645522
TATACAC8500.032.928813
TGGTATC12750.032.1267172
GCCGTCT7500.031.65036246
Produced by FastQC (version 0.11.5)